Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

doi:10.1055/s-0032-1328596

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	Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
	Zeng, Feng 1,2; Wang, Wei 3,4; Guan, Shuhong2 ; Cheng, Chunru 2; Yang, Min 2; Avula, Bharathi 5; Khan, Ikhlas A.5; Guo, De-an1,2
刊名	PLANTA MEDICA
	2013-06
卷号	79 期号:9 页码:797-805
关键词	Tripterygium wilfordii sesquiterpenoid alkaloids diterpenoids triterpenoids HPLC-ESI-MS multiple reaction monitoring Celastraceae
ISSN号	0032-0943
DOI	10.1055/s-0032-1328596
文献子类	Article
英文摘要	A liquid chromatography-electrospray ionization-mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C-18 column (150 x 4.6 mm, 3.5 mu m) with gradient elution using acetonitrile and 0.03% formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor-product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intra-day and interday variations of 2-5% and 1-4%, respectively, and recoveries of 95.5-104.5%.
资助项目	program for Changjiang Scholars and Innovation Team in University[985-2-063-112] ; Ministry of Education of China[104218] ; Global Research Network for Medicinal Plants (GRNMP)[00000000] ; King Saud University[00000000]
WOS关键词	SOLID-PHASE EXTRACTION ; CHINESE HERBAL REMEDY ; THUNDER GOD VINE ; NF-KAPPA-B ; HOOK-F ; ROOT EXTRACTS ; TRIPTOLIDE ; EXPRESSION ; SUPPRESSION ; ALKALOIDS
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	GEORG THIEME VERLAG KG
WOS记录号	WOS:000320451500013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277600]
专题	上海中药现代化研究中心
通讯作者	Guo, De-an
作者单位	1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; 2.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Biol Sci,Shanghai Inst Mat Med, Shanghai, Peoples R China; 3.Hunan Univ Chinese Med, Sch Pharm, TCM, Changsha 410208, Hunan, Peoples R China; 4.Hunan Univ Chinese Med, Sch Pharm, Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China; 5.Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, Sino US TCM Res Ctr, University, MS 38677 USA
推荐引用方式 GB/T 7714	Zeng, Feng,Wang, Wei,Guan, Shuhong,et al. Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry[J]. PLANTA MEDICA,2013,79(9):797-805.
APA	Zeng, Feng.,Wang, Wei.,Guan, Shuhong.,Cheng, Chunru.,Yang, Min.,...&Guo, De-an.(2013).Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry.PLANTA MEDICA,79(9),797-805.
MLA	Zeng, Feng,et al."Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry".PLANTA MEDICA 79.9(2013):797-805.