Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry

doi:10.1002/pca.2454

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	Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry
	Ling, Yun; Li, Zhixiong; Chen, Mingcang; Sun, Zhaolin; Fan, Mingsong; Huang, Chenggang
刊名	PHYTOCHEMICAL ANALYSIS
	2013-11
卷号	24 期号:6 页码:677-688
关键词	HPLC-QTOF/MS/MS chemical constituents Cong-Ming-Tang traditional Chinese medicine formula
ISSN号	0958-0344
DOI	10.1002/pca.2454
文献子类	Article
英文摘要	IntroductionCong-Ming-Tang (CMT), named smart-soup in English, is a well-known traditional Chinese medicine formula for the treatment of amnesia in China. However, the isolation, purification and identification procedures of the major bioactive constituents in CMT are difficult and time consuming. ObjectiveTo establish a rapid and sensitive high-performance liquid chromatography/electrospray ionisation with quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF/MS/MS) method that could be applied to rapidly separate and identify the major bioactive constituents in CMT. MethodsMethanolic extract of CMT was used for HPLC-QTOF/MS/MS analysis. Separation was performed on an Agilent Poroshell 120 EC- C18 column (2.7 x100 mm .i.d., 2.7 mu m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Both positive and negative ion modes were employed. ResultsThis analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retention times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phenylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules. ConclusionHPLC-QTOF/MS/MS provides a new powerful approach to identify the major chemical constituents in CMT rapidly and accurately. This study proposed a series of potential bioactive components without preparative isolation from the crude extract of CMT and indicated that the HPLC-QTOF/MS/MS method also can be a promising tool for the analysis of other traditional Chinese medicines. Copyright (c) 2013 John Wiley & Sons, Ltd. This analytical tool allowed the identification of 55 compounds from CMT formulae by comparing their retentionylpropanoids. Onjisaponin MF was tentatively elucidated as a new triterpene saponin based on the summarised fragmentation rules. n times and MS spectra with those of authentic compounds or literature data in both positive and negative ion modes, including 4 xanthone C-glycosides, 4 sucrose esters, 11 oligosaccharide multi-esters,15 triterpene saponins, 15 triterpene acids, 2 lignans and 4 phe.
资助项目	National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2012ZX09301001-001] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; Key Program of the Science and Technology Commission of Shanghai Municipality, China[09JC1416400]
WOS关键词	POLYGALA-TENUIFOLIA WILLD ; PORIA-COCOS ; TRITERPENE SAPONINS ; SURFACE-LAYER ; GLYCOSIDES ; ROOTS ; ESTERS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000326032100022
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277408]
专题	上海中药现代化研究中心
通讯作者	Huang, Chenggang
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Ling, Yun,Li, Zhixiong,Chen, Mingcang,et al. Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):677-688.
APA	Ling, Yun,Li, Zhixiong,Chen, Mingcang,Sun, Zhaolin,Fan, Mingsong,&Huang, Chenggang.(2013).Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry.PHYTOCHEMICAL ANALYSIS,24(6),677-688.
MLA	Ling, Yun,et al."Analysis of Multiple Constituents in Cong-Ming-Tang, a Chinese Herbal Formula for the Treatment of Amnesia, by High-performance Liquid Chromatography with Quadrupole Time-of-flight Mass Spectrometry".PHYTOCHEMICAL ANALYSIS 24.6(2013):677-688.