Metabolite identification of crude extract from Ganoderma lucidum in rats using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry

doi:10.1016/j.jchromb.2013.10.006

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	Metabolite identification of crude extract from Ganoderma lucidum in rats using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
	Cheng, Chun-Ru 1,2; Yang, Min 2; Yu, Kate 3; Guan, Shu-Hong2 ; Wu, Xiao-Hui 4; Wu, Wan-Ying2 ; Sun, Yan 2; Li, Chuan2 ; Ding, Jie 1; Guo, De-An2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2013-12-15
卷号	941 页码:90-99
关键词	Ganoderma lucidum Ganoderic acid Metabolism Metabolite identification UPLC/Q-TOF
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2013.10.006
文献子类	Article
英文摘要	The metabolism of traditional Chinese medicine is very complicated and has been a great challenge. In the present paper, a new strategy was established to study the metabolism of crude extract from Ganoderma lucidum using the highly separative and sensitive ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Based on the investigation of the metabolism of five representative single compounds (ganoderic acid), a total of 90 metabolites were identified from the bile sample after oral administration of the crude extract. Among them, 21 compounds were identified by comparison with the reference standards, the other unknown metabolites were tentatively characterized by interpretation of the high resolution low collision energy and high collision energy mass spectra using the fragmentation rules. The metabolic characteristics and "soft spots" of the ganoderic acids were revealed. After being absorbed, the ganoderic acids from the extract could undergo extensive phases land II metabolism in rat before excreted into the bile. The main ganoderic acids could transform from one to another through reduction, oxidation, deacetylation and desaturation reactions. Other metabolic transformation included hydroxylation, sulfation and glucuronidation. The total tendency was that the low polar ganoderic acids were transformed into the high polar metabolites to eliminate from the organism. The metabolic "soft spots" of the ganoderic acids were 3,7,15,23-carbonyl groups (or hydroxyl groups), angular methyl groups, 20(22)-double bond, 12-acetoxyl group and 26-carboxylic acid moiety. These results are considered to be important for the further investigation of G. lucidum. (C) 2013 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Discipline Construction Project of Sichuan University of Science Engineering[00000000] ; Project of Education Department in Sichuan[13ZB0133] ; Introduction Program of Scientific Researchist of Sichuan University of Science Engineering[2012RC17] ; Introduction Program of Scientific Researchist of Sichuan University of Science Engineering[2012RC15]
WOS关键词	ACID-D ; TRITERPENOIDS ; CYTOTOXICITY ; MUSHROOM ; HPLC
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000327367100013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277336]
专题	上海中药现代化研究中心
通讯作者	Yang, Min
作者单位	1.Sichuan Univ Sci & Engn, Sch Chem & Pharmaceut Engn, Inst Pharmaceut Engn Technol & Applicat, Zigong 643000, Sichuan, Peoples R China; 2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Waters Corp, Milford, MA 01757 USA; 4.Tianjin Med Univ, Tianjin Key Lab Technol Enabling Dev Clin Therape, Coll Pharm, Tianjin 300070, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Chun-Ru,Yang, Min,Yu, Kate,et al. Metabolite identification of crude extract from Ganoderma lucidum in rats using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,941:90-99.
APA	Cheng, Chun-Ru.,Yang, Min.,Yu, Kate.,Guan, Shu-Hong.,Wu, Xiao-Hui.,...&Guo, De-An.(2013).Metabolite identification of crude extract from Ganoderma lucidum in rats using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,941,90-99.
MLA	Cheng, Chun-Ru,et al."Metabolite identification of crude extract from Ganoderma lucidum in rats using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 941(2013):90-99.