TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways involved in the cardiotoxicity induced by Venenum Bufonis in rats

doi:10.1038/srep22759

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	TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways involved in the cardiotoxicity induced by Venenum Bufonis in rats
	Bi, Qi-rui 1,2; Hou, Jin-jun1 ; Qi, Peng 1; Ma, Chun-hua 2; Feng, Rui-hong 1; Yan, Bing-peng 1,2; Wang, Jian-wei 1,2; Shi, Xiao-jian 1,2; Zheng, Yuan-yuan 1; Wu, Wan-ying1
刊名	SCIENTIFIC REPORTS
	2016-03-10
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep22759
文献子类	Article
英文摘要	Venenum Bufonis (VB) is a widely used traditional medicine with serious cardiotoxic effects. The inflammatory response has been studied to clarify the mechanism of the cardiotoxicity induced by VB for the first time. In the present study, Sprague Dawley (SD) rats, were administered VB (100, 200, and 400 mg/kg) intragastrically, experienced disturbed ECGs (lowered heart rate and elevated ST-segment), increased levels of serum indicators (creatine kinase (CK), creatine kinase isoenzyme-MB (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST)) and serum interleukin (IL-6, IL-1 beta, TNF-alpha) at 2 h, 4 h, 6 h, 8 h, 24 h, and 48 h, which reflected that an inflammatory response, together with cardiotoxicity, were involved in VB-treated rats. In addition, the elevated serum level of MDA and the down-regulated SOD, CAT, GSH, and GPx levels indicated the appearance of oxidative stress in the VB-treated group. Furthermore, based on the enhanced expression levels of TXNIP, p-NF-kappa Bp65, p-I kappa B alpha, p-IKK alpha, p-IKK beta, p-ERK, p-JNK, and p-P38 and the obvious myocardial degeneration, it is proposed that VB-induced cardiotoxicity may promote an inflammatory response through the TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways. The observed inflammatory mechanism induced by VB may provide a theoretical reference for the toxic effects and clinical application of VB.
资助项目	National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Traditional Chinese Medicine Industry Research Special Project[201307002] ; Standardization of Traditional Chinese Medicines/Indigenous drugs - Chinese Academy of Sciences, China[KSZD-EW-Z-004]
WOS关键词	DOXORUBICIN-INDUCED CARDIOTOXICITY ; THIOREDOXIN-INTERACTING PROTEIN ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINE ; OXIDATIVE STRESS ; TOAD VENOM ; CARDIAC-GLYCOSIDES ; MASS-SPECTROMETRY ; P38 MAPK ; IN-VITRO
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000371684000001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276102]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-ying; Guo, De-an
作者单位	1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Haike Rd 501, Shanghai 201203, Peoples R China; 2.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Bi, Qi-rui,Hou, Jin-jun,Qi, Peng,et al. TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways involved in the cardiotoxicity induced by Venenum Bufonis in rats[J]. SCIENTIFIC REPORTS,2016,6.
APA	Bi, Qi-rui.,Hou, Jin-jun.,Qi, Peng.,Ma, Chun-hua.,Feng, Rui-hong.,...&Guo, De-an.(2016).TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways involved in the cardiotoxicity induced by Venenum Bufonis in rats.SCIENTIFIC REPORTS,6.
MLA	Bi, Qi-rui,et al."TXNIP/TRX/NF-kappa B and MAPK/NF-kappa B pathways involved in the cardiotoxicity induced by Venenum Bufonis in rats".SCIENTIFIC REPORTS 6(2016).