Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF

doi:10.1016/j.jep.2016.03.049

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	Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF
	Bi, Qi-rui 1,2; Hou, Jin-jun2 ; Qi, Peng 2; Ma, Chun-hua 1; Shen, Yao 2; Feng, Rui-hong 2; Yan, Bing-peng 1,2; Wang, Jian-wei 1,2; Shi, Xiao-jian 1,2; Zheng, Yuan-yuan 2
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2016-06-20
卷号	186 页码:103-110
关键词	Brain-derived neurotrophic factor (BDNF) Inflammation Neurotoxicity NF NF- < kappa > B pathway Venenum Bufonis
ISSN号	0378-8741
DOI	10.1016/j.jep.2016.03.049
文献子类	Article
英文摘要	Ethnopharmacological relevance: Venenum Bufonis (VB), also called toad venom, has been widely used in clinic as a cardiotonic, anohyne and antineoplastic agents both in China and other Asian countries. However, its neurotoxicity and cardiotoxicity limit its wide clinical application. Compared with extensive attention attracted with cardiotoxicity, the toxic effect of VB on Central Nervous System (CNS) is much less studied. Aim of the research: This study was performed to examine the neurotoxicity caused by VB on Sprague Dawley (SD) rats, then to clarify the mechanism in vivo by investigating its action on the neuroinflammation which possibly attributed to the activation of nuclear factor-kappa B (NF-kappa B) pathway and the attenuation of brain-derived neurotrophic factor (BDNF). Materials and methods: Rats administrated with 0.5% carboxymethyl cellulose sodium salt (CMC-Na) aqueous solution and VB (100 mg/kg, 200 mg/kg and 400 mg/kg) were sacrificed at 2 h, 4 h, 6 h, 8 h, 24 h and 48 h. The brain level of neurotransmitters and their corresponding receptors, pro-inflammatory cytokines, BDNF/TrkB and NF-kappa B pathway-related proteins were examined, respectively. Results: VB administration induced severe neurologic damage and neuroinflammation, as indicated by the disordered 5-hydroxytryptamine (5-HT), dopamine (DA) and their corresponding receptors, together with the over production of inflammatory cytokines including interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). VB also notably promoted the expression of p-NF-kappa Bp65, p-I kappa B alpha, p-IKK alpha and p-IKK beta and down-regulated the expression of BDNF and TrkB. Conclusion: This study demonstrates that VB triggers neurotoxicity which probably is induced by neuroinflammation via activating of NF-kappa B pathway and attenuating the expression of BDNF. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
资助项目	National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Traditional Chinese Medicine Industry Research Special Project[201307002] ; Standardization of Traditional Chinese Medicines/Indigenous drugs - Chinese Academy of Sciences, China[KSZD-EW-Z-004-01]
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; HIPPOCAMPAL-NEURONS ; CHINESE-MEDICINE ; NEUROTROPHIC FACTOR ; MASS-SPECTROMETRY ; CORTICAL-NEURONS ; 5 BUFADIENOLIDES ; NERVOUS-SYSTEM ; TOAD VENOM ; BRAIN
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000377832000011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275993]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-ying; Guo, Dean
作者单位	1.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Bi, Qi-rui,Hou, Jin-jun,Qi, Peng,et al. Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF[J]. JOURNAL OF ETHNOPHARMACOLOGY,2016,186:103-110.
APA	Bi, Qi-rui.,Hou, Jin-jun.,Qi, Peng.,Ma, Chun-hua.,Shen, Yao.,...&Guo, Dean.(2016).Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF.JOURNAL OF ETHNOPHARMACOLOGY,186,103-110.
MLA	Bi, Qi-rui,et al."Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF".JOURNAL OF ETHNOPHARMACOLOGY 186(2016):103-110.