Colon-derived uremic biomarkers induced by the acute toxicity of Kansui radix: A metabolomics study of rat plasma and intestinal contents by UPLC-QTOF-MSE

doi:10.1016/j.jchromb.2015.09.022

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	Colon-derived uremic biomarkers induced by the acute toxicity of Kansui radix: A metabolomics study of rat plasma and intestinal contents by UPLC-QTOF-MSE
	Yang, Zhou 1,2; Hou, Jin-Jun1 ; Qi, Peng 1; Yang, Min 1; Yan, Bing-Peng 1; Bi, Qi-Rui 1; Feng, Rui-Hong 1; Yang, Wen-Zhi 1; Wu, Wan-Ying1 ; Guo, De-An1
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2016-07-15
卷号	1026 页码:193-203
关键词	Kansui radix Metabolomics Toxicity Intestinal contents Colon-derived uremic biomarkers
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2015.09.022
文献子类	Article
英文摘要	Kansui radix (KR) is a poisonous Chinese herbal medicine recorded in the Chinese Pharmacopoeia, and the acute toxicity obstructs its clinical applications. To explore its acute toxicity mechanism to enhance clinical safety, a metabolomics study based on UPLC-ESI-QTOF-MSE was performed. Wistar rats were exposed for 4h to the aqueous and ethyl acetate extracts prepared from KR at a high dose (25 g/kg). The contents of six different sections of rat intestine, including the duodenum, jejunum, ileum, cecum, colon, and rectum were collected as samples for the first time, as well as the rat plasma. The interesting results showed that only those rats exposed to the ethyl acetate extract showed a watery diarrhea, similar to the observed acute human toxicity. The identified biomarkers found in the plasma, such as phenol sulfate, indoxyl sulfate, and p-cresol sulfate were significantly perturbed in the rats. These biomarkers are known as colon-derived uremic compounds, which were first reported with respect to KR. The three essential amino acids which produced these biomarkers were only found in the contents of colon and rectum. A hypothesis was proposed that only the colon-derived uremic compounds induced by KR might be responsible for the acute toxicity. Three traditional process methods to reduce the toxicity of KR were compared based on these biomarkers, and different levels of toxicity modulation were observed. These results may be helpful to further understand the mechanism of acute toxicity, and the relevance of the traditional process methods to ameliorate the adverse effects of KR. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81073026] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Standardization of Traditional Chinese Medicines/Indigenous drugs - Chinese Academy of Sciences-China[KSZD-EW-Z-004-01] ; National High Technology Research and Development Program of China[2013AA093002] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002]
WOS关键词	EUPHORBIA-KANSUI ; MASS-SPECTROMETRY ; METABONOMICS ; ROOTS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000379636800022
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275956]
专题	上海中药现代化研究中心
通讯作者	Wu, Wan-Ying; Guo, De-An
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Cailun Rd 1200, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Yang, Zhou,Hou, Jin-Jun,Qi, Peng,et al. Colon-derived uremic biomarkers induced by the acute toxicity of Kansui radix: A metabolomics study of rat plasma and intestinal contents by UPLC-QTOF-MSE[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1026:193-203.
APA	Yang, Zhou.,Hou, Jin-Jun.,Qi, Peng.,Yang, Min.,Yan, Bing-Peng.,...&Guo, De-An.(2016).Colon-derived uremic biomarkers induced by the acute toxicity of Kansui radix: A metabolomics study of rat plasma and intestinal contents by UPLC-QTOF-MSE.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1026,193-203.
MLA	Yang, Zhou,et al."Colon-derived uremic biomarkers induced by the acute toxicity of Kansui radix: A metabolomics study of rat plasma and intestinal contents by UPLC-QTOF-MSE".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1026(2016):193-203.