Electrophysiological and morphological properties of pyramidal and nonpyramidal neurons in the cat motor cortex in vitro

doi:10.1016/0306-4522(96)00009-7

	Electrophysiological and morphological properties of pyramidal and nonpyramidal neurons in the cat motor cortex in vitro
	Chen, W; Zhang, JJ; Hu, GY; Wu, CP
刊名	NEUROSCIENCE
	1996-07
卷号	73 期号:1 页码:39-55
关键词	regular-spiking doubler-or-burst firing narrow-spiking fast-spiking spine nonpyramidal neuron
ISSN号	0306-4522
DOI	10.1016/0306-4522(96)00009-7
文献子类	Article
英文摘要	Electrophysiological and morphological properties of the neurons in cat motor cortex were investigated using intracellular recording and staining techniques in a brain slice preparation. In response to intracellular injection of depolarizing current pulses, four distinct types of firing patterns were observed among cat neocortical neurons. Regular-spiking neurons were characterized by their repetitive firing from which conspicuous frequency adaptation was observed. Doublet-or-burst firing cells were marked with their tendency to fire 2-5 clustered spikes at the onset of depolarizing pulse. In doublet-or-burst firing neurons, but not in regular-spiking neurons, a low-threshold calcium current was revealed by single-electrode voltage clamp. Both regular-spiking and doublet-or-burst firing neurons had relatively wide action potentials. Fast-spiking neurons could fire extremely narrow action potentials at a very high frequency. Their frequency-to-intensity slope of steady-state firing was significantly higher than that of the other neurons. In contrast, narrow-spiking neurons had the smallest frequency-to-intensity slope for steady-state firing, although their action potentials were as narrow as those of the fast-spiking neurons. Both regular-spiking and doublet-or-burst firing neurons were identified as pyramidal neurons, and were found in all layers below layer I. Their apical dendrites were densely coated with dendritic spines. Narrow-spiking neurons were only recorded in layer V. They were large pyramidal cells with scarce spines on their apical dendrites. Fast-spiking neurons were all nonpyramidal interneurons. Seven out of eight labelled fast-spiking cells had beaded dendrites without spines. Their axons had a large number of varicosities, and arborized extensively to form a dense plexus of terminals in the vicinity of their soma. The remaining neuron was found to be a spiny nonpyramidal neuron in layer V. These results demonstrate that, in addition to the three types of firing patterns previously identified in rodent neocortex, a group of neurons in the cat motor cortex express another type of firing behaviour which is characterized by extremely narrow action potential and very small frequency-to-intensity slope. Correlation with the morphological data shows that these neurons are large layer V pyramidal cells rather than nonpyramidal interneurons. (C) 1996 IBRO. Published by Elsevier Science Ltd.
WOS关键词	INTRINSIC FIRING PATTERNS ; RAT VISUAL-CORTEX ; SENSORIMOTOR CORTEX ; CALCIUM CURRENTS ; NEOCORTICAL NEURONS ; SYNAPTIC RESPONSES ; INVITRO ; CELLS ; EXCITATION ; INHIBITION
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:A1996UN71000004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274970]
专题	科研条件处
作者单位	CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI BRAIN RES INST,SHANGHAI 200031,PEOPLES R CHINA
推荐引用方式 GB/T 7714	Chen, W,Zhang, JJ,Hu, GY,et al. Electrophysiological and morphological properties of pyramidal and nonpyramidal neurons in the cat motor cortex in vitro[J]. NEUROSCIENCE,1996,73(1):39-55.
APA	Chen, W,Zhang, JJ,Hu, GY,&Wu, CP.(1996).Electrophysiological and morphological properties of pyramidal and nonpyramidal neurons in the cat motor cortex in vitro.NEUROSCIENCE,73(1),39-55.
MLA	Chen, W,et al."Electrophysiological and morphological properties of pyramidal and nonpyramidal neurons in the cat motor cortex in vitro".NEUROSCIENCE 73.1(1996):39-55.