A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma

doi:10.1002/bmc.773

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	A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma
	Tang, Yi-hong; Zhang, Yuan-yuan; Zhu, Hai-yan; Huang, Cheng-gang
刊名	BIOMEDICAL CHROMATOGRAPHY
	2007-05
卷号	21 期号:5 页码:458-462
关键词	saikosaponin a solid-phase extraction HPLC
ISSN号	0269-3879
DOI	10.1002/bmc.773
文献子类	Article
英文摘要	A high-performance liquid chromatographic method with UV detection has been developed for the determination of saikosaponin a in rat plasma. Saikosaponin a and internal standard jujuboside A were isolated from plasma samples by solid-phase extraction. The chromatographic separation was achieved on a reversed-phase C, column with the mobile phase of acetonitrile-water (35:65, v/v) at a flow rate of 1 mL/min and UV detection was set at 205 nm. The standard curve for saikosaponin a was linear over the concentration range 0.25-10 mu g/mL and the limit of detection was 0.05 mu g/mL. The absolute recovery was greater than 82%. The precision and accuracy ranged from 3.05 to 9.59% and 95.61 to 110.00%, respectively. The validated method was used to determine saikosaponin a in plasma samples in a pharmacokinetic study of saikosaponin a administered to Sprague-Dawley rats. Copyright (c) 2007 John Wiley & Sons, Ltd.
WOS关键词	BUPLEURUM-FALCATUM ; ASSAY ; RADIX
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	JOHN WILEY & SONS LTD
WOS记录号	WOS:000246161200004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273264]
专题	上海中药现代化研究中心
通讯作者	Huang, Cheng-gang
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tang, Yi-hong,Zhang, Yuan-yuan,Zhu, Hai-yan,et al. A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma[J]. BIOMEDICAL CHROMATOGRAPHY,2007,21(5):458-462.
APA	Tang, Yi-hong,Zhang, Yuan-yuan,Zhu, Hai-yan,&Huang, Cheng-gang.(2007).A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma.BIOMEDICAL CHROMATOGRAPHY,21(5),458-462.
MLA	Tang, Yi-hong,et al."A high-performance liquid chromatographic method for saikosaponin a quantification in rat plasma".BIOMEDICAL CHROMATOGRAPHY 21.5(2007):458-462.