Quantitative determination of 14 major constituents in the herbal preparation Luan-Pao-Prescription using HPLC coupled with photodiode array detection

doi:10.1365/s10337-007-0296-5

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	Quantitative determination of 14 major constituents in the herbal preparation Luan-Pao-Prescription using HPLC coupled with photodiode array detection
	Zhang, Jin-qiang ; Wang, Xiao-ming ; Lu, Zhi-qiang ; Huang, Hui-lian ; Chen, Guang-tong ; Liu, Rong-xia ; Bi, Kai-Shun ; Guo, De-an
刊名	CHROMATOGRAPHIA
	2007-08
卷号	66 期号:3-4 页码:267-270
关键词	column liquid chromatography Luan-pao-prescription quality control traditional Chinese medicine
ISSN号	0009-5893
DOI	10.1365/s10337-007-0296-5
文献子类	Article
英文摘要	High performance liquid chromatography coupled with photocliode array detection (HPLC-DAD) was employed for the determination of 14 major constituents in traditional Chinese herbal formula 'Luan-Pao-Prescription'. Two different chromatographic determinations for the 14 constituents were carried out to control the quality of this herbal formula. The sample was extracted under ultrasonic extraction with 75% methanol at the frequency of 42 kHz for 45 min at room temperature. The chromatographic separation was performed on a Zorbax SB-C-18 .The mobile phase was linear gradient elution of water with 0.5% (v/v) acetic acid (A) and acetonitrile (B) column with linear gradient elution procedure. The flow rate was set at 1.0 mL min(-1) and the column temperature was kept at 30 degrees C. The calibration curve for the determination of all the analytes showed good linearity over the investigated ranges (R-2 > 0.999). The intra- and inter-day assay precision (RSD) of 14 analytes were in the range of 0.2-3.9% and the recoveries were between 92.4 and 104.4%. The validated method was successfully applied to the determination of 14 major constituents in LPP.
WOS关键词	SCUTELLARIA-BAICALENSIS-GEORGI ; OXIDATIVE STRESS ; LYMPHOCYTES ; FLAVONOIDS ; INHIBITION ; BAICALIN ; CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	VIEWEG
WOS记录号	WOS:000249232900021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273185]
专题	上海中药现代化研究中心
通讯作者	Guo, De-an
作者单位	1.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Green Valley Res Inst,Shanghai Res Ctr Modernizat, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jin-qiang,Wang, Xiao-ming,Lu, Zhi-qiang,et al. Quantitative determination of 14 major constituents in the herbal preparation Luan-Pao-Prescription using HPLC coupled with photodiode array detection[J]. CHROMATOGRAPHIA,2007,66(3-4):267-270.
APA	Zhang, Jin-qiang.,Wang, Xiao-ming.,Lu, Zhi-qiang.,Huang, Hui-lian.,Chen, Guang-tong.,...&Guo, De-an.(2007).Quantitative determination of 14 major constituents in the herbal preparation Luan-Pao-Prescription using HPLC coupled with photodiode array detection.CHROMATOGRAPHIA,66(3-4),267-270.
MLA	Zhang, Jin-qiang,et al."Quantitative determination of 14 major constituents in the herbal preparation Luan-Pao-Prescription using HPLC coupled with photodiode array detection".CHROMATOGRAPHIA 66.3-4(2007):267-270.