A feasible, economical, and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways

doi:10.1016/S1875-5364(17)30048-1

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	A feasible, economical, and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways
	Zhang Yi-Bei ; Da Juan ; Zhang Jing-Xian ; Li Shang-Rong ; Chen Xin ; Long Hua-Li ; Wang Qiu-Rong ; Cai Lu-Ying ; Yao Shuai ; Hou Jin-Jun
刊名	CHINESE JOURNAL OF NATURAL MEDICINES
	2017-04
卷号	15 期号:4 页码:301-309
关键词	Aconiti Lateralis Radix Praeparata Single Standard for Determination of Multi-Components (SSDMC) Monoester-diterpenoidaconitines (MDAs) Diester-diterpenoidaconitines (DDAs)
ISSN号	2095-6975
DOI	10.1016/S1875-5364(17)30048-1
文献子类	Article
英文摘要	Aconiti Lateralis Radix Praeparata (Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines (MDAs) and diester-diterpenoidaconitines (DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components (SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate (recovery varying between 97.5%-101.8%, RSD < 3%), precise (RSD 0.63%-2.05%), and linear (R > 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice (GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis (PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii (prepared parent root) and Aconitum kusnezoffii (prepared root).
资助项目	project of Standardization of Traditional Chinese Medicines/Indigenous Drugs - Chinese Academy of Sciences[KSZD-EW-Z-004-01]
WOS关键词	TANDEM MASS-SPECTROMETRY ; SINGLE ; METABOLOMICS ; TOXICITY ; ROOTS ; FUZI
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5988711
出版者	CHINESE JOURNAL NATURAL MEDICINES
WOS记录号	WOS:000402468900008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272712]
专题	上海中药现代化研究中心
通讯作者	Wu Wan-Ying
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Tradit Chinese Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Zhang Yi-Bei,Da Juan,Zhang Jing-Xian,et al. A feasible, economical, and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2017,15(4):301-309.
APA	Zhang Yi-Bei.,Da Juan.,Zhang Jing-Xian.,Li Shang-Rong.,Chen Xin.,...&Guo De-An.(2017).A feasible, economical, and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways.CHINESE JOURNAL OF NATURAL MEDICINES,15(4),301-309.
MLA	Zhang Yi-Bei,et al."A feasible, economical, and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways".CHINESE JOURNAL OF NATURAL MEDICINES 15.4(2017):301-309.