A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study

doi:10.1007/s00216-017-0604-3

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	A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study
	Su, Chong 2; Sun, Hui 2; Yang, Hong 2; Yin, Lei 2; Zhang, Jiwen3 ; Fawcett, John Paul 1; Gu, Jingkai 2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2017-11
卷号	409 期号:28 页码:6559-6565
关键词	Ion exchange solid phase extraction LC-MS/MS Porcine relaxin Ultrahigh performance liquid chromatography
ISSN号	1618-2642
DOI	10.1007/s00216-017-0604-3
文献子类	Article
英文摘要	Porcine relaxin is a 6 kDa peptide hormone of pregnancy with important physiological and pharmacological effects. It contains a number of analogs of which porcine relaxin B29 is one of the most important. To support the development of porcine relaxin B29 as a new drug, we established an UPLC-MS/MS method for its quantitation in dog plasma. Sample preparation by protein precipitation and ion exchange solid phase extraction was followed by UPLC on an XBridge (TM) BEH300 C18 column at 40 A degrees C in a run time of only 5.5 min. Detection was performed on a Qtrap 6500 mass spectrometer using ESI in the positive ion mode with MRM of the transitions at m/z 831.7 [M+7H](7+) -> 505.4 and m/z 1162.4 [M+5H](5+) -> 226 for pRLX B29 and internal standard (recombinant human insulin), respectively. The method was linear over the concentration range 30-2000 ng/mL with no matrix effects. Intra- and inter-day precisions were < 15% with accuracies in the range 98.8-100.6%. The method was successfully applied to a pharmacokinetic study in beagle dogs after administration of a 0.15 mg/kg intravenous dose.
资助项目	National Natural Science Foundation of China[81430087] ; National Natural Science Foundation of China[81473142] ; National Natural Science Foundation of China[81673396] ; National Natural Science Foundation of China[81673502] ; National Natural Science Foundation of China[81603182]
WOS关键词	CHROMATOGRAPHY-MASS SPECTROMETRY ; RECOMBINANT HUMAN RELAXIN-2 ; IMMUNOAFFINITY PURIFICATION ; HIGH RESOLUTION ; INSULIN ; QUANTIFICATION ; PEPTIDES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000414329900004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272426]
专题	药物制剂研究中心
通讯作者	Gu, Jingkai
作者单位	1.Univ Otago, Sch Pharm, POB 56, Dunedin 9054, New Zealand 2.Jilin Univ, Sch Life Sci, 2699 Qinajin Rd, Changchun 130012, Jilin, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Su, Chong,Sun, Hui,Yang, Hong,et al. A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6559-6565.
APA	Su, Chong.,Sun, Hui.,Yang, Hong.,Yin, Lei.,Zhang, Jiwen.,...&Gu, Jingkai.(2017).A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6559-6565.
MLA	Su, Chong,et al."A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6559-6565.