Development of a liquid chromatographic-tandem mass spectrometric method with precolumn derivatization for the determination of dencichine in rat plasma

doi:10.1016/j.aca.2006.03.011

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	Development of a liquid chromatographic-tandem mass spectrometric method with precolumn derivatization for the determination of dencichine in rat plasma
	Zhang, Y ; Chen, XY; Li, XY ; Zhong, DF
刊名	ANALYTICA CHIMICA ACTA
	2006-05-04
卷号	566 期号:2 页码:200-206
关键词	liquid chromatography-tandem mass spectrometry dencichine rat plasma
ISSN号	0003-2670
DOI	10.1016/j.aca.2006.03.011
文献子类	Article
英文摘要	Dencichine is a nonprotein amino acid used for hemostasis. A very sensitive method was developed for the determination of dencichine concentration in rat plasma using a derivatization step to enhance signal intensity. The method consisted of a protein precipitation extraction followed by derivatization with 10 M hydrochloric acid-methanol (10:90, v/v) and analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS) via atmospheric pressure chemical ionization (APCI) source. The separation was achieved using a XDB C-8 analytical column with an isocratic mobile phase composed of methanol-water-formic acid in the ratio of 35:65:0.5 (v/v/v). Signal intensity of the dencichine derivative was increased up to 50-fold as compared to the underivatized dencichine in positive APCI mode. The matrix effect was also investigated. The method has a lower limit of quantification of 20 ng/ml for a 100 mu l plasma aliquot. The intra- and inter-day precision (R.S.D.), calculated from quality control (QC) samples, was less than 6.3%. The accuracy (R.E.), determined using QC samples, ranged from -0.2 to 3.9%. The method offered increased sensitivity, selectivity and speed of analysis over existing method. The method was utilized to support clinical pharmacokinetic studies of dencichine in rat followina intravenous administration. It indicated that this LC-MS-MS method is useful for pharmacokinetic studies of dencichine in animals, because it enabled the serial determination of plasma concentration of dencichine in rats. (c) 2006 Elsevier B.V. All rights reserved.
WOS关键词	NEUROTOXIN BETA-ODAP ; LATHYRUS-SATIVUS ; BETA-N-OXALYL-L-ALPHA,BETA-DIAMINOPROPIONIC ACID ; ALPHA-ISOMER
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000237522700008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273601]
专题	上海药物代谢研究中心
通讯作者	Zhong, DF
作者单位	1.Shenyang Pharmaceut Univ, Lab Drug Metab & Pharmacokinet, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Metab & Pharmacokinet Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Y,Chen, XY,Li, XY,et al. Development of a liquid chromatographic-tandem mass spectrometric method with precolumn derivatization for the determination of dencichine in rat plasma[J]. ANALYTICA CHIMICA ACTA,2006,566(2):200-206.
APA	Zhang, Y,Chen, XY,Li, XY,&Zhong, DF.(2006).Development of a liquid chromatographic-tandem mass spectrometric method with precolumn derivatization for the determination of dencichine in rat plasma.ANALYTICA CHIMICA ACTA,566(2),200-206.
MLA	Zhang, Y,et al."Development of a liquid chromatographic-tandem mass spectrometric method with precolumn derivatization for the determination of dencichine in rat plasma".ANALYTICA CHIMICA ACTA 566.2(2006):200-206.