Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry

doi:10.1016/j.jchromb.2008.02.002

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	Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry
	Liu, Ke; Dai, Xiaojian; Zhong, Dafang; Chen, Xiaoyan
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2008-03-15
卷号	864 期号:1-2 页码:129-136
关键词	ondansetron enantiomers stereoselective pharmacokinetics enantioselective LC-MS/MS
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2008.02.002
文献子类	Article
英文摘要	A sensitive and enantioselective method was developed and validated for the determination of ondansetron enantiomers in human plasma using enantioselective liquid chromatography-tandem mass spectrometry. The enantiomers of ondansetron were extracted from plasma using ethyl acetate under alkaline conditions. HPLC separation was performed on an ovomucoid column using an isocratic mobile phase of methanol-5 MM ammonium acetate-acetic acid (20:80:0.02, v/v/v) at a flow rate of 0.40 mL/min. Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode, using the transitions of m/z 294 -> 170 for ondansetron enantiomers, and m/z 285 -> 124 for tropisetron (internal standard). The method was linear in the concentration range of 0. 10-40 ng/mL for each enantiomer using 200 mu L of plasma. The lower limit of quantification (LLOQ) for each enantiomer was 0. 10 ng/mL. The intra- and inter-assay precision was 3.7-11.6% and 5.6-12.3 % for R-(-)-ondansetron and S-(+)ondansetron, respectively. The accuracy was 100.4-107. 1 % for R-(-)-ondansetron and 103.3-104.9% for S-(+)-ondansetron. No chiral inversion was observed during the plasma storage, preparation and analysis. The method was successfully applied to characterize the pharmacokinetic profiles of ondansetron enantiomers in healthy volunteers after an intravenous infusion of 8 mg racemic ondansetron. (C) 2008 Published by Elsevier B.V.
WOS关键词	SOLID-PHASE EXTRACTION ; CHIRAL STATIONARY-PHASE ; POSTOPERATIVE NAUSEA ; ENANTIOMERS ; PHARMACOKINETICS ; METABOLITES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000254774400018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272959]
专题	上海药物代谢研究中心
通讯作者	Chen, Xiaoyan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ke,Dai, Xiaojian,Zhong, Dafang,et al. Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,864(1-2):129-136.
APA	Liu, Ke,Dai, Xiaojian,Zhong, Dafang,&Chen, Xiaoyan.(2008).Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,864(1-2),129-136.
MLA	Liu, Ke,et al."Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 864.1-2(2008):129-136.