Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study

doi:10.1016/j.jchromb.2008.08.024

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	Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study
	Ma, Jinfei 2,3; Chen, Xiaoyan2 ; Duan, Xiaotao 2; Deng, Pan 2; Wang, Hui 1; Zhong, Dafang2
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2008-10-01
卷号	873 期号:2 页码:203-208
关键词	Liquid chromatography-tandem mass spectrometry Rasagiline Pharmacokinetics
ISSN号	1570-0232
DOI	10.1016/j.jchromb.2008.08.024
文献子类	Article
英文摘要	A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed to determine rasagiline in human plasma. The analytical method utilized liquid-liquid extraction of plasma with n-hexane-dichloromethane-isopropanol (20:10:1, v/v/v). Separation of analyte and the internal standard (IS) pseudoephedrine was performed on a Zorbax Extend C-18 column (150 mm x 4.6 mm, 5 mu m) with a mobile phase consisting of acetonitrile-5 mM ammonium acetate-acetic acid (40:60:0.05, v/v/v) at a flow rate of 0.5 mL/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring mode via positive electrospray ionization interface using the transitions m/z 172.1 --> m/z (117.1 + 115.1) for rasagiline, and m/z 166.0 --> m/z 148.1 for the internal standard. The method was linear over the concentration range of 0.020-50.0 ng/mL. The intra- and inter-day precisions were less than 11.2% in terms of relative standard deviation (R.S.D.), and the accuracy was within +/- 6.4% in terms of relative error (RE), The lower limit of quantification (LLOQ) was identifiable and reproducible at 0.020 ng/mL with acceptable precision and accuracy. The mean extraction-efficiency at three concentrations was 95.6 +/- 7.0%, 97.9 +/- 3.0% and 95.3 +/- 8.3%, The validated method offered increased sensitivity (10 times higher than those reported) and wide linear concentration range. This method was successfully applied for the evaluation of pharmacokinetics of rasagiline after single oral doses of 1.2 and 5 mg rasagiline to 12 Chinese healthy volunteers. (C) 2008 Elsevier BY. All rights reserved.
WOS关键词	MONOAMINE-OXIDASE-B ; PARKINSONS-DISEASE ; MASS-SPECTROMETRY ; SELEGILINE ; INHIBITOR ; DESMETHYLSELEGILINE ; DERIVATIVES ; POTENT
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000260196000011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272792]
专题	上海药物代谢研究中心
通讯作者	Zhong, Dafang
作者单位	1.Chongqing Pharmaceut Res Inst Co Ltd, Chongqing 400061, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Zhejiang Univ Technol, Hangzhou 310014, Zhejiang, Peoples R China;
推荐引用方式 GB/T 7714	Ma, Jinfei,Chen, Xiaoyan,Duan, Xiaotao,et al. Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,873(2):203-208.
APA	Ma, Jinfei,Chen, Xiaoyan,Duan, Xiaotao,Deng, Pan,Wang, Hui,&Zhong, Dafang.(2008).Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,873(2),203-208.
MLA	Ma, Jinfei,et al."Validated LC-MS/MS method for quantitative determination of rasagiline in human plasma and its application to a pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 873.2(2008):203-208.