Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential

doi:10.1007/s10719-017-9804-5

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential
	Pan, Yongxi 3; Wang, Peipei2 ; Zhang, Fuming 4; Yu, Yanlei 3; Zhang, Xing 3; Lin, Lei 3; Linhardt, Robert J.1,3,4
刊名	GLYCOCONJUGATE JOURNAL
	2018-02
卷号	35 期号:1 页码:87-94
关键词	Glycosaminoglycans Fish maw Compositional analysis Chondroitin sulfate Heparan sulfate Disaccharides FGF-2
ISSN号	0282-0080
DOI	10.1007/s10719-017-9804-5
文献子类	Article
英文摘要	The swim bladder of fish is an internal gas-filled organ that allows fish to control their buoyancy and swimming depth. Fish maws (the dried swim bladders of fish) have been used over many centuries as traditional medicines, tonics and a luxurious gourmet food in China and Southeast Asia. Little is known about the structural information of polysaccharides comprising this important functional material of fish tissue. In the present study, the total glycosaminoglycan (GAG) from fish maw was characterized. Two GAGs were identified, chondroitin sulfate (CS, having a molecular weight of 18-40 kDa) and heparan sulfate (HS), corresponding to 95% and 5% of the total GAG, respectively. Chondroitinase digestion showed that the major CS GAG was composed of Delta UA-1 -> 3-GalNAc4S (59.7%), Delta UA-1 -> 3-GalNAc4,6S (36.5%), Delta UA-1 -> 3-GalNAc6S (2.2%) and Delta UA-1 -> 3-GalNAc (1.6%) disaccharide units. H-1-NMR analysis and degradation with specific chondroitinases, both CS-type A/C and CS-type B were present in a ratio of 1.4:1. Analysis using surface plasmon resonance showed that fibroblast growth factor (FGF)-2 bound to the CS fraction (K-D = 136 nM). These results suggest that this CS may be involved in FGF-signal pathway, mediating tissue repair, regeneration and wound healing. The CS, as the major GAG in fish maw, may have potential pharmacological activity in accelerating wound healing.
资助项目	National Institutes of Health[HL125371] ; National Institutes of Health[GM38060] ; National Institutes of Health[HL096972] ; National Institutes of Health[HL062244] ; National Institutes of Health[HL136271]
WOS关键词	LARGE YELLOW CROAKER ; CHONDROITIN SULFATE ; HEPARIN ; BIOLOGY ; CROCEA ; ACID
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000427484000009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279917]
专题	药理学第三研究室国家新药筛选中心
通讯作者	Wang, Peipei; Zhang, Fuming; Linhardt, Robert J.
作者单位	1.Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Biol, Biomed Engn, Troy, NY 12180 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 3.Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Chem & Chem Biol, Troy, NY 12180 USA; 4.Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Chem & Biol Engn, Troy, NY 12180 USA;
推荐引用方式 GB/T 7714	Pan, Yongxi,Wang, Peipei,Zhang, Fuming,et al. Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential[J]. GLYCOCONJUGATE JOURNAL,2018,35(1):87-94.
APA	Pan, Yongxi.,Wang, Peipei.,Zhang, Fuming.,Yu, Yanlei.,Zhang, Xing.,...&Linhardt, Robert J..(2018).Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential.GLYCOCONJUGATE JOURNAL,35(1),87-94.
MLA	Pan, Yongxi,et al."Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential".GLYCOCONJUGATE JOURNAL 35.1(2018):87-94.