Structural characterization of a galactan from Ophiopogon japonicus and anti-pancreatic cancer activity of its acetylated derivative

doi:10.1016/j.ijbiomac.2018.03.019

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural characterization of a galactan from Ophiopogon japonicus and anti-pancreatic cancer activity of its acetylated derivative
	Gu, Dan 2,3,4; Huang, Lulin 2,4; Chen, Xia 2,4; Wu, Qianhu 1,3; Ding, Kan2,4
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-07-01
卷号	113 页码:907-915
关键词	Ophiopogon japonicus Polysaccharide Acetylated derivative Apoptosis Anti-pancreatic cancer
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.03.019
文献子类	Article
英文摘要	A galactan ROH05 was isolated from the roots of Ophiopogon japonicus and further purified by DEAE Sepharose (TM) Fast Flow columns. The molecular weight of ROH05 was estimated to be 16.7 kDa. ROH05 was composed of galactose only. According to the methylation analysis and the results of IR and NMR spectra, the backbone of ROH05 was composed of 1, 4-linked beta-D-galactose and 1, 4, 6-linked beta-D-galactose, while the branch of this polysaccharide was terminal-linked beta-D-galactose attached at C-6 position of 1, 4-linked p-D-galactose directly. The acetylated galactan, ROHO5A was prepared by the acetic anhydride-pyridine method. The acetylation modification mainly occurred at C-2, C-3 and C-6 positions of I, 4-linked beta-D-galactose, and C-6 position of terminal-linked S-D-galactose. Bioactivity test showed that ROHO5A might inhibit both BxPC-3 and PANC-1 pancreatic cancer cells growth in a dose-dependent manner without significant toxicity to LO2 cells. In addition, the cells apoptosis was triggered since cleaved caspase-3 and FasL were activated while p53, p21, Bax expression were up-regulated by ROHO5A as well. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	New Drug Creation and Manufacturing Program[2017ZX09305-003] ; Ministry of Science and Technology, the People Republic of China[00000000] ; State Key Program of National Natural Science Foundation of China[31230022] ; Program of Shanghai Subject Chief Scientist[16XD1404500] ; Science and Technology Planning Program of Zhenjiang, Science and Technology Board of Zhenjiang[SH2014061]
WOS关键词	ANTIOXIDANT ACTIVITY ; AUGMENTS SURVIVAL ; ISCHEMIC-HEART ; POLYSACCHARIDE ; MDG-1 ; MICE ; ANGIOGENESIS ; EXPRESSION ; FLOWERS ; S1P(1)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000432503100104
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279693]
专题	药理学第三研究室
通讯作者	Wu, Qianhu; Ding, Kan
作者单位	1.Jiangsu Univ, Hosp Zhenjiang 3, 300 Daijiamen, Zhenjiang 212001, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 3.Nanjing Univ Chinese Med, Coll Pharm, 138 Xianlin Ave, Nanjing 210029, Jiangsu, Peoples R China; 4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Gu, Dan,Huang, Lulin,Chen, Xia,et al. Structural characterization of a galactan from Ophiopogon japonicus and anti-pancreatic cancer activity of its acetylated derivative[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,113:907-915.
APA	Gu, Dan,Huang, Lulin,Chen, Xia,Wu, Qianhu,&Ding, Kan.(2018).Structural characterization of a galactan from Ophiopogon japonicus and anti-pancreatic cancer activity of its acetylated derivative.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,113,907-915.
MLA	Gu, Dan,et al."Structural characterization of a galactan from Ophiopogon japonicus and anti-pancreatic cancer activity of its acetylated derivative".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 113(2018):907-915.