Structural characterization of a pectic polysaccharide from Nerium indicum flowers

doi:10.1016/j.phytochem.2010.05.019

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural characterization of a pectic polysaccharide from Nerium indicum flowers
	Dong, Qun; Liu, Xuan; Yao, Jian; Dong, Xiaotang; Ma, Chao; Xu, Yuxia; Fang, Jinian; Ding, Kan
刊名	PHYTOCHEMISTRY
	2010-08
卷号	71 期号:11-12 页码:1430-1437
关键词	Nerium indicum Apocynaceae Oleander Flower Pectic polysaccharide
ISSN号	0031-9422
DOI	10.1016/j.phytochem.2010.05.019
文献子类	Article
英文摘要	A polysaccharide fraction, J6, was isolated from the hot-water extract of flowers of oleander Nerium indicum Mill., using ethanol precipitation, cetyltrimethylammonium bromide (CTAB) complexing, anion-exchange chromatography and gel permeation chromatography. J6 was found to contain L-rhamnose, L-arabinose, D-galactose, and D-galacturonic acid, in the ratio of 10.1:49.8:30.1:10.0. Its structure was investigated by methylation analysis, periodate oxidation, Smith degradation, partial acid hydrolysis, electrospray ionization mass spectrometry and NMR spectroscopic methods. It was found that J6 is an RG-I type polysaccharide, which contains a rhamnogalacturonan backbone, with various branches attached to O-4 of L-rhamnose. The branches probably involve (1 -> 4)-beta-D-galactan, branched L-arabino-(1 -> 3)(1 -> 6)-beta-D-galactan, and (1 -> 5)-alpha-L-arabinan. J6 stimulated NO production of macrophage RAW264.7 cells in a preliminary test. (C) 2010 Elsevier Ltd. All rights reserved.
资助项目	Chinese Academy of Sciences, China[00000000] ; Chinese Academy of Sciences[KSCX1-YW-R-18] ; National Natural Science Foundation of China (NSFC)[30670470] ; National High Technology Research and Development Program (863) of China[2006AA02Z102] ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences[07G603E036]
WOS关键词	NITRIC-OXIDE ; RHAMNOGALACTURONAN-I ; SIDE-CHAINS ; CELL-WALLS ; OLEANDER ; FEATURES ; IMMUNOMODULATION ; ARABINOGALACTAN ; ACTIVATION ; PROTEINS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000280321000029
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278823]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Dong, Qun,Liu, Xuan,Yao, Jian,et al. Structural characterization of a pectic polysaccharide from Nerium indicum flowers[J]. PHYTOCHEMISTRY,2010,71(11-12):1430-1437.
APA	Dong, Qun.,Liu, Xuan.,Yao, Jian.,Dong, Xiaotang.,Ma, Chao.,...&Ding, Kan.(2010).Structural characterization of a pectic polysaccharide from Nerium indicum flowers.PHYTOCHEMISTRY,71(11-12),1430-1437.
MLA	Dong, Qun,et al."Structural characterization of a pectic polysaccharide from Nerium indicum flowers".PHYTOCHEMISTRY 71.11-12(2010):1430-1437.