Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus

doi:10.1128/JB.06582-11

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus
	Deng, Xin 1,2; Sun, Fei 1,2; Ji, Quanjiang 1,2; Liang, Haihua 1,2; Missiakas, Dominique 3; Lan, Lefu4 ; He, Chuan 1,2
刊名	JOURNAL OF BACTERIOLOGY
	2012-04
卷号	194 期号:7 页码:1753-1762
ISSN号	0021-9193
DOI	10.1128/JB.06582-11
文献子类	Article
英文摘要	Staphylococcus aureus utilizes efflux transporter NorA to pump out a wide range of structurally dissimilar drugs, conferring low-level multidrug resistance. The regulation of norA expression has yet to be fully understood although past studies have revealed that this gene is under the control of the global transcriptional regulator MgrA and the two-component system ArlRS. To identify additional regulators of norA, we screened a transposon library in strain Newman expressing the transcriptional fusion norA-lacZ for altered beta-galactosidase activity. We identify a transposon insertion in fhuB, a gene that encodes a ferric hydroxamate uptake system permease, and propose that the norA transcription is iron responsive. In agreement with this observation, addition of FeCl3 repressed the induction of norA-lacZ, suggesting that bacterial iron uptake plays an important role in regulating norA transcription. In addition, a fur (ferric uptake regulator) deletion exhibited compromised norA transcription and reduced resistance to quinolone compared to the wild-type strain, indicating that fur functions as a positive regulator of norA. A putative Fur box identified in the promoter region of norA was confirmed by electrophoretic mobility shift and DNase I footprint assays. Finally, by employing a siderophore secretion assay, we reveal that NorA may contribute to the export of siderophores. Collectively, our experiments uncover some novel interactions between cellular iron level and norA regulation in S. aureus.
资助项目	NIH[R01 AI074658] ; Region V Great Lakes Regional Center of Excellence in Biodefense and Emerging Infectious Diseases Consortium (National Institute of Allergy and Infectious Diseases)[1-U54-AI-057153]
WOS关键词	FERRIC-UPTAKE REGULATOR ; HELICOBACTER-PYLORI ; POSITIVE REGULATION ; VIBRIO-VULNIFICUS ; MOLECULAR CHARACTERIZATION ; PSEUDOMONAS-AERUGINOSA ; SIDEROPHORE EXPORT ; VIRULENCE FACTORS ; ESCHERICHIA-COLI ; GENOME SEQUENCE
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000301344700013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278143]
专题	药理学第三研究室
通讯作者	He, Chuan
作者单位	1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 3.Univ Chicago, Dept Microbiol, Chicago, IL 60637 USA; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Deng, Xin,Sun, Fei,Ji, Quanjiang,et al. Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus[J]. JOURNAL OF BACTERIOLOGY,2012,194(7):1753-1762.
APA	Deng, Xin.,Sun, Fei.,Ji, Quanjiang.,Liang, Haihua.,Missiakas, Dominique.,...&He, Chuan.(2012).Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus.JOURNAL OF BACTERIOLOGY,194(7),1753-1762.
MLA	Deng, Xin,et al."Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus".JOURNAL OF BACTERIOLOGY 194.7(2012):1753-1762.