Magnesium lithospermate B inhibits intraceHular calcium elevation induced by ATP and KCl in rat vascular smooth muscle cells

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	Magnesium lithospermate B inhibits intraceHular calcium elevation induced by ATP and KCl in rat vascular smooth muscle cells
	TIAN Xia; WANG Yiping
刊名	Chinese Pharmacological Bulletin
	2006
卷号	22 期号:3 页码:311-316
关键词	Fluo-3 magnesium lithospermate B vasoconstric-tion vascular smooth muscle cells intracellular freecalcium Fluo-3
ISSN号	1001-1978
其他题名	丹酚酸B镁抑制ATP和KCl诱导大鼠主动脉平滑肌细胞内钙的升高
文献子类	Article
英文摘要	Aim To investigate the effects of magnesium lithospermate B （ MLB ） on vasoconstriction of denuded aortic rings in MLB on cytosolic vitro, as well as the effects of （[Ca^2＋ ]i） in aortic （AVSMCs）. Methods in the rat thoracic aortic free calcium concentration vascular smooth muscle cells Isotonic tension was measured rings without endothelium and quantitative changes of [ Ca^2＋ ]i were directly monitored in AVSMCs loaded with the fluorescent calcium indicator Fluo-3 using intracellular cation measurement system. tension Results MLB had no effects on resting ring in the presence or absence of extracellular Ca^2＋. In rings exposed to 50 ～200 mumol · L^-1 MLB, the isotonic contractions induced by 1 mumol · L^-1 PE were decreased in calcium-free K-H solution. However, in the presence of extracellular Ca^2＋ , KCl 60 mmol·L^-1 produced isotonic contractions that were abolished by verapamil 10 mumol · L^-1 or inhibited by pretreatment of 50～200 mumol · L^-1 MLB in a concentration-dependent manner. When administered to the rings precontracted with 1 mumol · L^-1 PE in the absence of extracellular Ca^2 ＋ , a second extracelluar-calcium-dependent vasoconstriction induced by restoration of extracellular Ca2＋ was also reduced by pretreatment of 50～200 mumol · L^-1 MLB. Moreover, MLB, at concentration of 50 ～200 mumol · L^-1, produced little effect on AVSMCs [ Ca^2＋ ] i in the presence or absence of extracellular Ca^2＋ . In AVSMCs exposed to 50 ～200 mumol · L^-1MLB, the inhibitory effects of MLB on [ Ca^2＋ ]i induced by 20mumol · L^-1 ATP were concentration-dependent, with decreases ranging from 17.4% to 61.1% in the absence of extracellular Ca^2＋. In the presence of extracellular Ca^2＋ and thapsigargin, the increases in AVSMCs [ Ca^2＋ ] i evoked by 60 mumol · L^-1 KCl were inhibited by pretreatment with MLB （50 ～ 200 mumol · L^-1） or abolished by pretreatment with verapamil （10 mumol · L^-1）. Conclusions MLB inhibits vasoconstriction induced by PE, high-K^＋ , and re-Ca^2＋. MLB also has inhibitory effects on increase of [ Ca^2＋]i induced by ATP and high K^＋ in AVSMCs. These results indicate that both the blocking effects on calcium release and voltage-dependent calcium channels may be responsible for the effects of MLB on [ Ca^2＋ ]i in vascular smooth muscle cells.
资助项目	国家十五重大科技专项创新药物和中药现代化资助项目[00000000]
WOS研究方向	General & Internal Medicine (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2343455
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268210]
专题	药理学第一研究室
通讯作者	WANG Yiping
作者单位	State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.
推荐引用方式 GB/T 7714	TIAN Xia,WANG Yiping. Magnesium lithospermate B inhibits intraceHular calcium elevation induced by ATP and KCl in rat vascular smooth muscle cells[J]. Chinese Pharmacological Bulletin,2006,22(3):311-316.
APA	TIAN Xia,&WANG Yiping.(2006).Magnesium lithospermate B inhibits intraceHular calcium elevation induced by ATP and KCl in rat vascular smooth muscle cells.Chinese Pharmacological Bulletin,22(3),311-316.
MLA	TIAN Xia,et al."Magnesium lithospermate B inhibits intraceHular calcium elevation induced by ATP and KCl in rat vascular smooth muscle cells".Chinese Pharmacological Bulletin 22.3(2006):311-316.