Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry

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	Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry
	Yu Hualing 2; Chen Xiaoyan1 ; Zhu Lin 2; Zhong Dafang2
刊名	Chinese Pharmaceutical Journal
	2006
卷号	41 期号:6 页码:463-466
关键词	ilunarizine liquid chromatography-tandem mass spectrometry plasma concentration
ISSN号	1001-2494
其他题名	液相色谱-串联质谱法测定人血浆中氟桂利嗪
文献子类	Article
英文摘要	OBJECTIVE To develop a sensitive and specific LC/MS/MS method for the direct determination of ilunarizine in human plasma. METHODS Flunarizine and internal standard diphenhydramine were extracted from 0.25 mL plasma using liquid-liquid extraction, then separated on a Zorbax SB C18 column. The mobile phase consisted of acetonitrile-water-formic acid （80：20：0.5）. A Finnigan TSQ tandem mass spectrometer equipped with atmospheric pressure chemical ionization source was used as detector and was operated in the positive ion mode. Selected reaction monitoring （SRM） mode using the precursor to product the ion of m/z 405 to m/z 203 and m/z 256 to m/z 167 was performed to quantify flunarizine and the internal standard, respectively. RESULTS The linear calibration curves were obtained in the concentration range of 0.2 - 200.0mug·L^-1. The lower limit of quantification was 0.2mug·L^-1. The inter- and intra- day precision （RSD） were below 6.5%, and the accuracy （RE） was within 99.1% calculated from Qc samples. The method gave the mean recovery of 75.1% and 73.1% to ilunarizine and internal standard diphenhydramine, respectively. The total chromatographic run time was only 3.1 min. CONCLUSION The method is proved to be special, sensitive, and suitable for the clinical investigation of ilunarizine pharmacokinetics.
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:2578273
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268198]
专题	上海药物代谢研究中心
通讯作者	Zhong Dafang
作者单位	1.Research Center for Drug Metabolism, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. 2.Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.;
推荐引用方式 GB/T 7714	Yu Hualing,Chen Xiaoyan,Zhu Lin,et al. Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry[J]. Chinese Pharmaceutical Journal,2006,41(6):463-466.
APA	Yu Hualing,Chen Xiaoyan,Zhu Lin,&Zhong Dafang.(2006).Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry.Chinese Pharmaceutical Journal,41(6),463-466.
MLA	Yu Hualing,et al."Determination of Flunarizine in Human Plasma by Liquid Chromatography-tandem Mass Spectrometry".Chinese Pharmaceutical Journal 41.6(2006):463-466.