A sensitive LC-MS/MS method for the determination of triptolide and its application to pharmacokinetic research in rats

doi:10.1002/bmc.4422

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	A sensitive LC-MS/MS method for the determination of triptolide and its application to pharmacokinetic research in rats
	Xu, Ye; Chen, Xiaoyan; Zhong, Dafang
刊名	Biomedical chromatography : BMC
	2018-10-29
页码	e4422
ISSN号	1099-0801
DOI	10.1002/bmc.4422
文献子类	Article
英文摘要	Triptolide is one of the main active ingredients of Tripterygium wilfordii Hook. F. In this study, a sensitive LC-MS/MS method was established and validated to determine the concentration of triptolide in rat plasma. Triptolide and an internal standard [(5R)-5-hydroxytriptolide] were extracted from 100 μL of rat plasma with acetonitrile, and the dried residue was then reconstituted and reacted with benzylamine to produce benzylamine triptolide and benzylamine (5R)-5-hydroxytriptolide. Derivatization increased the sensitivity of triptolide detection by ~100-fold. Quantification was performed using a QTRAP 5500 tandem mass spectrometer with positive electrospray ionization in multiple reaction monitoring mode with an ion transition m/z 468.5 → 192.0 for benzylamine triptolide and m/z 484.3 → 192.1 for benzylamine (5R)-5-hydroxytriptolide. Good linearity was observed in the range of 0.030-100 ng/mL with a lower limit of quantitation of 0.030 ng/mL. The intra- and inter-day precision was <6.5%, and the accuracy ranged from -11.7 to -4.4%. The recovery remained consistent and was reproducible at different concentrations. This method was successfully applied to the study of triptolide drug-drug interactions in Sprague-Dawley rats. With the use of itraconazole (40 mg/kg, p.o.) as a CYP3A inhibitor, the plasma exposure of triptolide in rats was increased by 36%.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266732]
专题	上海药物代谢研究中心
作者单位	State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
推荐引用方式 GB/T 7714	Xu, Ye,Chen, Xiaoyan,Zhong, Dafang. A sensitive LC-MS/MS method for the determination of triptolide and its application to pharmacokinetic research in rats[J]. Biomedical chromatography : BMC,2018:e4422.
APA	Xu, Ye,Chen, Xiaoyan,&Zhong, Dafang.(2018).A sensitive LC-MS/MS method for the determination of triptolide and its application to pharmacokinetic research in rats.Biomedical chromatography : BMC,e4422.
MLA	Xu, Ye,et al."A sensitive LC-MS/MS method for the determination of triptolide and its application to pharmacokinetic research in rats".Biomedical chromatography : BMC (2018):e4422.