Proteome Analysis of Renoprotection Mediated by a Novel Cyclic Helix B Peptide in Acute Kidney Injury

doi:10.1038/srep18045

	Proteome Analysis of Renoprotection Mediated by a Novel Cyclic Helix B Peptide in Acute Kidney Injury
	Yang, Cheng 2,3,9; Liu, Junjun 1; Li, Long 3; Hu, Meiyu 3,4; Long, Yaqiu 5; Liu, Xiaohui 6; Zhu, Tongyu 2,3; Huang, Xiao 7; Zhao, Shouliang 1; Liu, Shangfeng 1
刊名	SCIENTIFIC REPORTS
	2015-12-10
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep18045
文献子类	Article
英文摘要	We developed a novel, erythropoietin-derived, non-erythropoiesis, cyclic helix B peptide (CHBP) that displays potent renoprotection against acute kidney injury (AKI). To determine the mechanism of CHBPmediated protection, we investigated the proteomic profile of mice treated with CHBP in a kidney ischemia-reperfusion (IR) injury model. The isobaric tags for relative and absolute quantitation (iTRAQ)labeled samples were analyzed using a QSTAR XL LC/MS system. In total, 38 differentially expressed proteins (DEPs) were shared by all experimental groups, while 3 DEPs were detected specifically in the IR + CHBP group. Eight significant pathways were identified, and oxidative phosphorylation was shown to be the most important pathway in CHBP-mediated renoprotection. The significant DEPs in the oxidative phosphorylation pathway elicited by CHBP are NADH-ubiquinone oxidoreductase Fe-S protein 6 (NDUFS6), alpha-aminoadipic semialdehyde synthase (AASS) and ATP-binding cassette subfamily D member 3 (ABCD3). The DEPs mentioned above were verified by RT-qPCR and immunostaining in mouse kidneys. We tested 6 DEPs in human biopsy samples from kidney transplant recipients. The trend of differential expression was consistent with that in the murine model. In conclusion, this study helps to elucidate the pharmacological mechanisms of CHBP before clinical translation.
资助项目	National Natural Science Foundation of China[81270832] ; National Natural Science Foundation of China[81270833] ; National Natural Science Foundation of China[81570674] ; National Natural Science Foundation of China[31401089] ; National Natural Science Foundation of China[81400752] ; China National Science Fund for Distinguished Young Scholars[81325020] ; Science and Technology Commission of Shanghai Municipality[12ZR1405500]
WOS关键词	RENAL ISCHEMIA/REPERFUSION INJURY ; ISCHEMIA-REPERFUSION INJURY ; LARGE GENE LISTS ; OXIDATIVE STRESS ; NETWORK ANALYSIS ; TUBULAR CELLS ; UP-REGULATION ; ERYTHROPOIETIN ; CALRETICULIN ; APOPTOSIS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000366130200001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276284]
专题	药物化学研究室
通讯作者	Liu, Shangfeng
作者单位	1.Fudan Univ, Huashan Hosp, Dept Stomatol, Shanghai 200040, Peoples R China; 2.Fudan Univ, Zhongshan Hosp, Dept Urol, Shanghai 200032, Peoples R China; 3.Shanghai Key Lab Organ Transplantat, Shanghai 200032, Peoples R China; 4.Fudan Univ, Zhongshan Hosp, Biomed Res Ctr, Shanghai 200032, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 200032, Peoples R China; 6.Fudan Univ, Inst Biomed Sci, Dept Chem, Shanghai 200433, Peoples R China; 7.Tongji Hosp, Tongji Hosp, Translat Ctr Stem Cell Res, Sch Med, Shanghai 200065, Peoples R China; 8.Fudan Univ, Zhongshan Hosp, Dept Transfus, Shanghai 200032, Peoples R China; 9.Fudan Univ, Zhongshan Hosp, Dept Plast Surg, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Yang, Cheng,Liu, Junjun,Li, Long,et al. Proteome Analysis of Renoprotection Mediated by a Novel Cyclic Helix B Peptide in Acute Kidney Injury[J]. SCIENTIFIC REPORTS,2015,5.
APA	Yang, Cheng.,Liu, Junjun.,Li, Long.,Hu, Meiyu.,Long, Yaqiu.,...&Rong, Ruiming.(2015).Proteome Analysis of Renoprotection Mediated by a Novel Cyclic Helix B Peptide in Acute Kidney Injury.SCIENTIFIC REPORTS,5.
MLA	Yang, Cheng,et al."Proteome Analysis of Renoprotection Mediated by a Novel Cyclic Helix B Peptide in Acute Kidney Injury".SCIENTIFIC REPORTS 5(2015).