A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

doi:10.3390/polym9060228

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative
	Liu, Yanqiu 1,4,5; Ding, Yaqi 1,3,5; Ye, Min 2; Zhu, Tao 4; Tian, Danbi 3; Ding, Kan1,5
刊名	POLYMERS
	2017-06
卷号	9 期号:6
关键词	Antrodia camphorata mannofucogalactan sulfated polysaccharide anti-angiogenesis
ISSN号	2073-4360
DOI	10.3390/polym9060228
文献子类	Article
英文摘要	A heterogalactan, named ACW0, was extracted from Antrodia camphorata and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 k Da. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of alpha-D-1,6-linked Gal, attached by a non-reducing terminal alpha-D-Man and alpha-L-Fuc on C-2 of nearly every six alpha-D-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development.
资助项目	State Key Program of National Natural Science Foundation of China[31230022] ; Program of Shanghai Subject Chief Scientist[16XD1404500]
WOS关键词	FOCAL ADHESION KINASE ; DISRUPTING ANGIOGENESIS ; CELL-PROLIFERATION ; CANCER-CELLS ; POLYSACCHARIDES ; GROWTH ; INHIBITION ; CINNAMOMEA ; ARABINOGALACTAN ; ELUCIDATION
WOS研究方向	Polymer Science
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000404218500043
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272637]
专题	药理学第三研究室
通讯作者	Tian, Danbi; Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, 38 Xueyuan Rd, Beijing 100191, Peoples R China 3.Nanjing Tech Univ, Dept Chem & Mol Engn, 30 Puzhu South Rd, Nanjing 211816, Jiangsu, Peoples R China; 4.Univ Sci & Technol China, Nano Sci & Technol Inst, 96 Jin Zhai Rd, Hefei 230026, Peoples R China; 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Yanqiu,Ding, Yaqi,Ye, Min,et al. A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative[J]. POLYMERS,2017,9(6).
APA	Liu, Yanqiu,Ding, Yaqi,Ye, Min,Zhu, Tao,Tian, Danbi,&Ding, Kan.(2017).A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative.POLYMERS,9(6).
MLA	Liu, Yanqiu,et al."A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative".POLYMERS 9.6(2017).