Glycoengineering and glycosite-specific labeling of serum IgGs from various species

doi:10.1016/j.carres.2017.05.001

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	Glycoengineering and glycosite-specific labeling of serum IgGs from various species
	Guo, Weilai 1,2; Tang, Feng 2,3; Qin, Ken 2,3; Zhou, Mang 2; Le, Zhiping 1; Huang, Wei 2,3
刊名	CARBOHYDRATE RESEARCH
	2017-06-29
卷号	446 页码:32-39
关键词	Chemoenzymatic glycoengineering Serum IgG Glycosite-specific IgG labeling Rabbit IgG Mouse IgG Goat IgG
ISSN号	0008-6215
DOI	10.1016/j.carres.2017.05.001
文献子类	Article
英文摘要	Chemoenzymatic glycoengineering of immunoglobulin G (IgG) catalyzed by Endo-S is a powerful approach to remodel the heterogeneous N-glycoforms of Fc domain with a homogeneous synthetic glycan structure for enhanced Fc receptor-mediated effector functions. The previous researches on the method development mainly focused on human or humanized IgGs with therapeutic potentials. Here, for the first time we report the extended application of this method on glycan-remodeling of serum IgGs from other species including rabbit, mouse, and goat. Harnessing an azido-tagged non-natural N-glycan substrate and successive click reaction, glycosite-specific fluorescent labeling of IgGs was enabled. This study provided a new avenue for glycoengineering and Fc-specific labeling of IgGs with minimized influence on antigen-binding domains, and this method was adaptive to thousands of commercial antibody reagents from various species with great application potentials. (C) 2017 Elsevier Ltd. All rights reserved,
资助项目	National Natural Science Foundation of China (NNSFC)[21572244] ; National Natural Science Foundation of China (NNSFC)[21372238] ; SIMM institute fund[CASIMM0120153004] ; "Personalized Medicines: Molecular Signature-based Drug Discovery and Development" (Strategic Priority Research Program of the Chinese Academy of Sciences)[XDA12020311] ; Innovation Project of Instrument and Equipment Function Development from Chinese Academy of Sciences[YZ201542]
WOS关键词	ENDOHEXOSAMINIDASE-CATALYZED GLYCOSYLATION ; FC-GAMMA-RIII ; CHEMOENZYMATIC SYNTHESIS ; THERAPEUTIC ANTIBODIES ; SIALIC ACIDS ; COPPER-FREE ; MALDI-MS ; ENDOGLYCOSIDASE ; GLYCOPROTEIN ; TRANSGLYCOSYLATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000404702700006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272597]
专题	生物技术药物研发中心（筹）药物安全性评价中心
通讯作者	Le, Zhiping; Huang, Wei
作者单位	1.Nanchang Univ, Dept Chem, 999 Xuefu Ave, Nanchang 330031, Jiangxi, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Ctr Excellence Mol Cell Sci, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Guo, Weilai,Tang, Feng,Qin, Ken,et al. Glycoengineering and glycosite-specific labeling of serum IgGs from various species[J]. CARBOHYDRATE RESEARCH,2017,446:32-39.
APA	Guo, Weilai,Tang, Feng,Qin, Ken,Zhou, Mang,Le, Zhiping,&Huang, Wei.(2017).Glycoengineering and glycosite-specific labeling of serum IgGs from various species.CARBOHYDRATE RESEARCH,446,32-39.
MLA	Guo, Weilai,et al."Glycoengineering and glycosite-specific labeling of serum IgGs from various species".CARBOHYDRATE RESEARCH 446(2017):32-39.