Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells

doi:10.3390/polym9090399

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells
	Jin, Can 1,2,3; Du, Zhenyun 2,3; Lin, Liyan 1,2,3; Zhou, Lishuang 1,2,3; Li, Saijuan 1,2,3; Liu, Qin 2,3; Ding, Kan1,2,3
刊名	POLYMERS
	2017-09
卷号	9 期号:9
关键词	mannoglucan polysaccharide Dendrobium nobile Lindl neuritogenesis neurodegeneration disease
ISSN号	2073-4360
DOI	10.3390/polym9090399
文献子类	Article
英文摘要	A water-soluble polysaccharide (JCS1) was isolated from the stems of Dendrobium nobile Lindl. JCS1 was structurally characterized using a combination of chemical and spectral analysis, including methylation analysis, partial acid hydrolysis, Fourier-transform infrared (FTIR) spectroscopy, gas chromatography (GC), GC-mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. The molecular weight was estimated to be 2.3 x 10(4) Da using high-performance gel permeation chromatography (HPGPC). The sugar composition analysis indicated it was composed of glucose, mannose, xylose, and arabinose in a 40.2:2.3:1.7:1.0 molar ratio. The structure analysis showed that JCS1 was a mannoglucan with a backbone consisting of (1 -> 4)-linked beta-Manp and (1 -> 4)-linked alpha-Glcp with branches at C-6 of (1 -> 4)-linked alpha-Glcp residues. The branches were composed of T-alpha-Glcp, 1,4-alpha-Xylp, and T-alpha-Araf. In vitro bioactivity tests revealed that the acetylated derivative of JCS1, YJCS1, induced neuritogenesis of PC-12 cells. These results demonstrate that YJCS1 might be a promising bioactive polysaccharide for development as a drug candidate for the possible prevention and treatment of neurodegeneration diseases.
资助项目	Project of the Shanghai Committee of Science and Technology[15495800300] ; State Key Program of National Natural Science Foundation of China[31230022] ; Program of Shanghai Subject Chief Scientist[16XD1404500] ; National Natural Science Foundation of China (NSFC)[31670814]
WOS关键词	WATER-SOLUBLE POLYSACCHARIDE ; ANTIOXIDANT ACTIVITIES ; IN-VITRO ; SELENYLATION MODIFICATION ; INONOTUS-OBLIQUUS ; STEMS ; HUOSHANENSE ; CARBOXYMETHYLATION ; BIOACTIVITY ; FRACTIONS
WOS研究方向	Polymer Science
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000411524400017
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272499]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	1.Zunyi Med Univ, Sch Pharm, 201 Dalian Rd, Zunyi 563003, Peoples R China; 2.Chinese Acad Sci, Shangshai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China; 3.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jin, Can,Du, Zhenyun,Lin, Liyan,et al. Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells[J]. POLYMERS,2017,9(9).
APA	Jin, Can.,Du, Zhenyun.,Lin, Liyan.,Zhou, Lishuang.,Li, Saijuan.,...&Ding, Kan.(2017).Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells.POLYMERS,9(9).
MLA	Jin, Can,et al."Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells".POLYMERS 9.9(2017).