Metabolomic Analysis of Anti-Hypoxia and Anti-anxiety Effects of Fu Fang Jin Jing Oral Liquid

doi:10.1371/journal.pone.0078281

	Metabolomic Analysis of Anti-Hypoxia and Anti-anxiety Effects of Fu Fang Jin Jing Oral Liquid
	Liu, Xia4 ; Zhu, Wei 1; Guan, Shuhong2 ; Feng, Ruihong 2; Zhang, Hui 1; Liu, Qiuhong 1; Sun, Peng 4; Lin, Donghai 3; Zhang, Naixia4 ; Shen, Jun 1
刊名	PLOS ONE
	2013-10-18
卷号	8 期号:10
ISSN号	1932-6203
DOI	10.1371/journal.pone.0078281
文献子类	Article
英文摘要	Background: Herba Rhodiolae is a traditional Chinese medicine used by the Tibetan people for treating hypoxia related diseases such as anxiety. Based on the previous work, we developed and patented an anti-anxiety herbal formula Fu Fang Jin Jing Oral Liquid (FJJOL) with Herba Rhodiolae as a chief ingredient. In this study, the anti-hypoxia and anti-anxiety effects of FJJOL in a high altitude forced-swimming mouse model with anxiety symptoms will be elucidated by NMR-based metabolomics. Methods: In our experiments, the mice were divided randomly into four groups as flatland group, high altitude saline-treated group, high altitude FJJOL-treated group, and high altitude diazepam-treated group. To cause anxiety effects and hypoxic defects, a combination use of oxygen level decreasing (hypobaric cabin) and oxygen consumption increasing (exhaustive swimming) were applied to mice. After a three-day experimental handling, aqueous metabolites of mouse brain tissues were extracted and then subjected to NMR analysis. The therapeutic effects of FJJOL on the hypobaric hypoxia mice with anxiety symptoms were verified. Results: Upon hypoxic exposure, both energy metabolism defects and disorders of functional metabolites in brain tissues of mice were observed. PCA, PLS-DA and OPLS-DA scatter plots revealed a clear group clustering for metabolic profiles in the hypoxia versus normoxia samples. After a three-day treatment with FJJOL, significant rescue effects on energy metabolism were detected, and levels of ATP, fumarate, malate and lactate in brain tissues of hypoxic mice recovered. Meanwhile, FJJOL also up-regulated the neurotransmitter GABA, and the improvement of anxiety symptoms was highly related to this effect. Conclusions: FJJOL ameliorated hypobaric hypoxia effects by regulating energy metabolism, choline metabolism, and improving the symptoms of anxiety. The anti-anxiety therapeutic effects of FJJOL were comparable to the conventional anti-anxiety drug diazepam on the hypobaric hypoxia mice. FJJOL might serve as an alternative therapy for the hypoxia and anxiety disorders.
资助项目	National Natural Science Foundation of China[30900662] ; Shanghai Foundation for Development of Science and Technology[08411965600] ; "100 Talents Program" of Chinese Academy of Sciences[00000000]
WOS关键词	MASS-SPECTROMETRY ; H-1-NMR SPECTROSCOPY ; OXIDATIVE STRESS ; PLUS-MAZE ; MICE ; RATS ; DEPRESSION ; METABOLITES ; ADAPTATION ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000326029300150
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277418]
专题	分析化学研究室药物安全性评价中心
通讯作者	Lin, Donghai
作者单位	1.Naval Med Res Inst, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 200031, Peoples R China; 3.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Chem Biol Fujian Prov, Xiamen, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai 200031, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Xia,Zhu, Wei,Guan, Shuhong,et al. Metabolomic Analysis of Anti-Hypoxia and Anti-anxiety Effects of Fu Fang Jin Jing Oral Liquid[J]. PLOS ONE,2013,8(10).
APA	Liu, Xia.,Zhu, Wei.,Guan, Shuhong.,Feng, Ruihong.,Zhang, Hui.,...&Shen, Jun.(2013).Metabolomic Analysis of Anti-Hypoxia and Anti-anxiety Effects of Fu Fang Jin Jing Oral Liquid.PLOS ONE,8(10).
MLA	Liu, Xia,et al."Metabolomic Analysis of Anti-Hypoxia and Anti-anxiety Effects of Fu Fang Jin Jing Oral Liquid".PLOS ONE 8.10(2013).