Spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase during mouse postnatal development

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	Spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase during mouse postnatal development
	Wang, LZ; Zhiu, XZ
刊名	ACTA PHARMACOLOGICA SINICA
	2003-10
卷号	24 期号:10 页码:965-974
关键词	D-amino acid oxidase serine serine racemase inbred C57BL mice
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To elucidate the spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase (EC 1.4.3.3; DAO) in mouse cortex, striatum, cerebellum, heart, lung, liver, spleen, kidney, and skeletal muscle during mouse postnatal development. METHODS: The transcription levels of serine racemase and DAO were assayed by reverse transcription-polymerase chain reaction (RT-PCR). The protein levels of serine racemase were examined by Western blot. DAO activities were assayed by colorimetric method. D-serine was measured by HPLC. RESULTS: In cortex, striatum, and cerebellum, free D-serine increased drastically after birth and coincided well with the increase of serine racemase expression. However, among the 9 tissues examined, DAO activities were detected only in cerebellum and kidney. During the 3rd week, DAO activity in cerebellum and kidney increased dramatically, which concurred with the drastic decline of D-serine content in these tissues. On the other hand, while D-serine and serine racemase fall to trace level in cerebellum and kidney at the 3rd weekend, DAO activities in these tissues increased continuously. CONCLUSION: The free D-serine is mainly synthesized by serine racemase. However, novel mechanisms might be involved in D-serine deposition in mouse tissues with high level of D-serine and no detectable DAO activity such as cortex and striatum. DAO in cerebellum and kidney might have other physiological functions in addition to degrading D-amino acid.
WOS关键词	D-ASPARTATE RECEPTOR ; RAT-BRAIN ; NMDA RECEPTORS ; EXTRACELLULAR CONCENTRATION ; SYNAPSE ELIMINATION ; GLYCINE SITE ; D-ALANINE ; NEUROMODULATOR ; CEREBELLUM ; RODENTS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	SHANGHAI INST MATERIA MEDICA
WOS记录号	WOS:000185749400003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274170]
专题	药理学第二研究室
通讯作者	Zhiu, XZ
作者单位	Chinese Acad Sci, Inst Biol Sci, Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Wang, LZ,Zhiu, XZ. Spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase during mouse postnatal development[J]. ACTA PHARMACOLOGICA SINICA,2003,24(10):965-974.
APA	Wang, LZ,&Zhiu, XZ.(2003).Spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase during mouse postnatal development.ACTA PHARMACOLOGICA SINICA,24(10),965-974.
MLA	Wang, LZ,et al."Spatiotemporal relationships among D-serine, serine racemase, and D-amino acid oxidase during mouse postnatal development".ACTA PHARMACOLOGICA SINICA 24.10(2003):965-974.