Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum

doi:10.1074/mcp.RA117.000372

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 化学蛋白质组学研究中心

	Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum
	Xu, Jun-Yu 1,2,3; Xu, Zhen 1,3; Liu, XinXin 3; Tan, Minjia2 ; Ye, Bang-Ce 1,3
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2018-06
卷号	17 期号:6 页码:1156-1169
关键词	Acetylation* Bacteria Enzyme Regulation* Mass Spectrometry Post-translational modifications* Quantification acylation regulation endospore-forming Clostridium metabolic shift quantitative acetylome quantitative butyrylome
ISSN号	1535-9476
DOI	10.1074/mcp.RA117.000372
文献子类	Article
英文摘要	Clostridium acetobutylicum is a strict anaerobic, endospore-forming bacterium, which is used for the production of the high energy biofuel butanol in metabolic engineering. The life cycle of C. acetobutylicum can be divided into two phases, with acetic and butyric acids being produced in the exponential phase (acidogenesis) and butanol formed in the stationary phase (solventogenesis). During the transitional phase from acidogenesis to solventogenesis and latter stationary phase, concentration peaks of the metabolic intermediates butyryl phosphate and acetyl phosphate are observed. As an acyl group donor, acyl-phosphate chemically acylates protein substrates. However, the regulatory mechanism of lysine acetylation and butyrylation involved in the phenotype and solventogenesis of C. acetobutylicum remains unknown. In our study, we conducted quantitative analysis of protein acetylome and butyrylome to explore the dynamic change of lysine acetylation and butyrylation in the exponential phase, transitional phase, and stationary phase of C. acetobutylicum. Total 458 lysine acetylation sites and 1078 lysine butyrylation sites were identified in 254 and 373 substrates, respectively. Bioinformatics analysis uncovered the similarities and differences between the two acylation modifications in C. acetobutylicum. Mutation analysis of butyrate kinase and the central transcriptional factor Spo0A was performed to characterize the unique role of lysine butyrylation in the metabolic pathway and sporulation process of C. acetobutylicum. Moreover, quantitative proteomic assays were performed to reveal the relationship between protein features (e.g. gene expression level and lysine acylation level) and metabolites in the three growth stages. This study expanded our knowledge of lysine acetylation and butyrylation in Clostridia and constituted a resource for functional studies on lysine acylation in bacteria.
WOS关键词	LYSINE ACETYLATION ; ESCHERICHIA-COLI ; MYCOBACTERIUM-TUBERCULOSIS ; ANALYSIS REVEALS ; GENE-EXPRESSION ; POSTTRANSLATIONAL MODIFICATION ; SACCHAROPOLYSPORA-ERYTHRAEA ; VIBRIO-PARAHEMOLYTICUS ; BACTERIAL CHEMOTAXIS ; MALONYLOME ANALYSIS
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000434203400009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279733]
专题	化学蛋白质组学研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ye, Bang-Ce
作者单位	1.Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Hangzhou 310014, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Xu, Jun-Yu,Xu, Zhen,Liu, XinXin,et al. Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum[J]. MOLECULAR & CELLULAR PROTEOMICS,2018,17(6):1156-1169.
APA	Xu, Jun-Yu,Xu, Zhen,Liu, XinXin,Tan, Minjia,&Ye, Bang-Ce.(2018).Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum.MOLECULAR & CELLULAR PROTEOMICS,17(6),1156-1169.
MLA	Xu, Jun-Yu,et al."Protein Acetylation and Butyrylation Regulate the Phenotype and Metabolic Shifts of the Endospore-forming Clostridium acetobutylicum".MOLECULAR & CELLULAR PROTEOMICS 17.6(2018):1156-1169.