Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products

doi:10.1016/j.chembiol.2018.05.005

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	Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products
	Xu, Jun-Yu 1,2,3; Xu, Ya 3; Xu, Zhen 3; Zhai, Lin-Hui 1; Ye, Yang1 ; Zhao, Yingming1,4 ; Chu, Xiaohe 2; Tan, Minjia1 ; Ye, Bang-Ce 2,3
刊名	CELL CHEMICAL BIOLOGY
	2018-08-16
卷号	25 期号:8 页码:984-+
ISSN号	2451-9448
DOI	10.1016/j.chembiol.2018.05.005
文献子类	Article
英文摘要	Coenzyme A (CoA) esters of short fatty acids (acyl-CoAs) function as key precursors for the biosynthesis of various natural products and the dominant donors for lysine acylation. Herein, we investigated the functional interplay between beneficial and adverse effects of acyl-CoA supplements on the production of acyl-CoA-derived natural products in microorganisms by using erythromycin-biosynthesized Saccharopolyspora erythraea as a model: accumulation of propionyl-CoA benefited erythromycin biosynthesis, but lysine propionylation inhibited the activities of important enzymes involved in biosynthetic pathways of erythromycin. The results showed that the overexpression of NAD(+)-dependent deacylase could circumvent the inhibitory effects of high acyl-CoA concentrations. In addition, we demonstrated the similar lysine acylation mechanism in other acyl-CoA-derived natural product biosynthesis, such as malonyl-CoA-derived alkaloid and butyryl-CoA-derived bioalcohol. These observations systematically uncovered the important role of protein acylation on interaction between the accumulation of high concentrations of acyl-CoAs and the efficiency of their use in metabolic pathways.
资助项目	National Natural Science Foundation of China[31730004] ; National Natural Science Foundation of China[21335003] ; National Natural Science Foundation of China[21575089] ; National Natural Science Foundation of China[91753203] ; Special Project on Precision Medicine under National Key RD Program[SQ2017YFSF090210]
WOS关键词	S-ADENOSYLMETHIONINE SYNTHETASE ; LYSINE ACETYLATION ; ESCHERICHIA-COLI ; SACCHAROPOLYSPORA-ERYTHRAEA ; QUANTITATIVE PROTEOMICS ; BACTERIAL PRODIGININES ; METABOLIC-REGULATION ; POLYKETIDE SYNTHASE ; STRAIN IMPROVEMENT ; SYSTEMS BIOLOGY
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000441791200008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279618]
专题	化学蛋白质组学研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Tan, Minjia; Ye, Bang-Ce
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gr, Hangzhou 310014, Zhejiang, Peoples R China; 3.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China; 4.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Xu, Jun-Yu,Xu, Ya,Xu, Zhen,et al. Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products[J]. CELL CHEMICAL BIOLOGY,2018,25(8):984-+.
APA	Xu, Jun-Yu.,Xu, Ya.,Xu, Zhen.,Zhai, Lin-Hui.,Ye, Yang.,...&Ye, Bang-Ce.(2018).Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products.CELL CHEMICAL BIOLOGY,25(8),984-+.
MLA	Xu, Jun-Yu,et al."Protein Acylation is a General Regulatory Mechanism in Biosynthetic Pathway of Acyl-CoA-Derived Natural Products".CELL CHEMICAL BIOLOGY 25.8(2018):984-+.