The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor

doi:10.1105/tpc.109.065730

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	The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor
	Yan, Jianbin ; Zhang, Chi 2; Gu, Min3 ; Bai, Zhiyan ; Zhang, Weiguo ; Qi, Tiancong ; Cheng, Zhiwei ; Peng, Wen ; Luo, Haibin 1; Nan, Fajun3
刊名	PLANT CELL
	2009-08
卷号	21 期号:8 页码:2220-2236
ISSN号	1040-4651
DOI	10.1105/tpc.109.065730
文献子类	Article
英文摘要	Jasmonates play a number of diverse roles in plant defense and development. CORONATINE INSENSITIVE1 (COI1), an F-box protein essential for all the jasmonate responses, interacts with multiple proteins to form the SCFCOI1 E3 ubiquitin ligase complex and recruits jasmonate ZIM-domain (JAZ) proteins for degradation by the 26S proteasome. To determine which protein directly binds to jasmonoyl-isoleucine (JA-Ile)/coronatine (COR) and serves as a receptor for jasmonate, we built a high-quality structural model of COI1 and performed molecular modeling of COI1-jasmonate interactions. Our results imply that COI1 has the structural traits for binding JA-Ile or COR. The direct binding of these molecules with COI1 was further examined using a combination of molecular and biochemical approaches. First, we used the immobilized jasmonate approach to show that the COI1 protein in crude leaf extracts can bind to the jasmonate moiety of JA-Ile. Second, we employed surface plasmon resonance technology with purified COI1 and JAZ1 protein to reveal the interaction among COI1, JA-Ile, and JAZ1. Finally, we used the photoaffinity labeling technology to show the direct binding of COR with purified insect-expressed COI1. Taken together, these results demonstrate that COI1 directly binds to JA-Ile and COR and serves as a receptor for jasmonate.
资助项目	National Science Foundation of China[00000000] ; The National Basic Research 973 Program of China[00000000] ; Chinese Academy of Sciences[00000000]
WOS关键词	METHYL JASMONATE ; PHYTOTOXIN CORONATINE ; UBIQUITIN-LIGASE ; AUXIN RECEPTOR ; BIOSYNTHESIS ; DEFENSE ; PATHWAY ; SIGNALS ; BINDING ; GROWTH
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000270416700006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279178]
专题	国家新药筛选中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Xie, Daoxin
作者单位	1.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China 2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Prot Sci Key Lab, Minist Educ,Sch Med, Beijing 100084, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Yan, Jianbin,Zhang, Chi,Gu, Min,et al. The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor[J]. PLANT CELL,2009,21(8):2220-2236.
APA	Yan, Jianbin.,Zhang, Chi.,Gu, Min.,Bai, Zhiyan.,Zhang, Weiguo.,...&Xie, Daoxin.(2009).The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor.PLANT CELL,21(8),2220-2236.
MLA	Yan, Jianbin,et al."The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor".PLANT CELL 21.8(2009):2220-2236.