Simultaneous determination of 24 constituents in Cortex Lycii using high-performance liquid chromatography-triple quadrupole mass spectrometry

doi:10.1016/j.jpba.2013.01.014

	Simultaneous determination of 24 constituents in Cortex Lycii using high-performance liquid chromatography-triple quadrupole mass spectrometry
	Zhang, Jing-Xian 1,2; Guan, Shu-Hong2 ; Yang, Min 2; Feng, Rui-Hong 2; Wang, Yang 1,2; Zhang, Yi-Bei 2; Chen, Xin 2; Chen, Xiao-Hui 1; Bi, Kai-Shun 1; Guo, De-An2
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2013-04-15
卷号	77 页码:63-70
关键词	HPLC-ESI-MS/MS Cortex Lycii Quantification Cyclic peptides Phenolic amides Principal component analysis
ISSN号	0731-7085
DOI	10.1016/j.jpba.2013.01.014
文献子类	Article
英文摘要	A fast high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry method was developed to determine 24 components including 11 phenolic compounds, 9 phenolic amides, and 4 cyclic peptides in Cortex Lycii. The analytes were quantified by a triple quadrupole instrument in multiple reaction monitoring (MRM) mode. The fragmentation patterns of phenolic amides and cyclic peptides using ESI and collision-induced dissociation (CID) techniques are reported. This assay method was validated with respect to linearity (r(2) > 0.9920), precision, repeatability, and accuracy (recovery rate between 93.0 and 105.9% with RSD < 4.4%). The analytical results of 28 batches of Cortex Lycii indicated that cyclic peptides and phenolic amides were not only the abundant constituents, but also the characteristic components for Cortex Lycii to distinguish from the adulterants. Principle component analysis (PCA) was used to discriminate samples from different geographical regions of China, and cyclic peptides were considered to be the chemical markers responsible for the classification. The systematic and integrated assessment of Cortex Lycii provides sufficient evidence for the establishment of the quality standard. (c) 2013 Elsevier B.V. All rights reserved.
资助项目	Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Global Research Network for Medicinal Plants (GRNMP)[00000000] ; King Saud University[00000000]
WOS关键词	DANGGUI-BUXUE-DECOCTION ; BIOACTIVE COMPONENTS ; CHINENSE ; RADICIS ; IDENTIFICATION ; PEPTIDES ; EXTRACTS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000316306000011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277655]
专题	成果转移转化处上海中药现代化研究中心
通讯作者	Guan, Shu-Hong
作者单位	1.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jing-Xian,Guan, Shu-Hong,Yang, Min,et al. Simultaneous determination of 24 constituents in Cortex Lycii using high-performance liquid chromatography-triple quadrupole mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,77:63-70.
APA	Zhang, Jing-Xian.,Guan, Shu-Hong.,Yang, Min.,Feng, Rui-Hong.,Wang, Yang.,...&Guo, De-An.(2013).Simultaneous determination of 24 constituents in Cortex Lycii using high-performance liquid chromatography-triple quadrupole mass spectrometry.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,77,63-70.
MLA	Zhang, Jing-Xian,et al."Simultaneous determination of 24 constituents in Cortex Lycii using high-performance liquid chromatography-triple quadrupole mass spectrometry".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 77(2013):63-70.