Shikonin inhibits tumor invasion via down-regulation of NF-kappa B-mediated MMP-9 expression in human ACC-M cells

doi:10.1111/j.1601-0825.2010.01758.x

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 新药研究国家重点实验室

	Shikonin inhibits tumor invasion via down-regulation of NF-kappa B-mediated MMP-9 expression in human ACC-M cells
	Min, R.1; Zun, Z.2; Min, Y.3; Wenhu, D.4; Wenjun, Y.1; Chenping, Z.1
刊名	ORAL DISEASES
	2011-05
卷号	17 期号:4 页码:362-369
关键词	Shikonin adenoid cystic carcinoma tumor invasion matrix metallopeptidase-9 nuclear factor-kappa B
ISSN号	1354-523X
DOI	10.1111/j.1601-0825.2010.01758.x
文献子类	Article
英文摘要	Objective: The aim of this study was to examine the anti-invasion effect of Shikonin on human high-metastatic adenoid cystic carcinoma (ACC-M) cells and to explain the possible molecular mechanism involved. Methods: The ACC-M cells were treated with Shikonin (0, 2.5, 5, 10 mu M) for 24 h. The protein levels and gelatinolytic activities of MMP-2 and MMP-9 were analyzed using Western blot and Gelatin zymography test, respectively. Matrigel invasion assays were used to investigate tumor invasive potential and electromobility shift assays were used to determine the activity of NF-kappa B. Results: The invasiveness of ACC-M cells was reduced in a dose dependent manner following 24-h treatment of up to 10 mu M of the Shikonin at which concentration no cytotoxicity occurred. The protein levels and gelatinolytic activities of MMP-9 were significantly suppressed by increasing Shikonin concentrations. The down-regulation of MMP-9 appeared to be via the inactivation of NF-kappa B as the treatment with Shikonin suppressed the protein level of phosphate-IkBa, which was accompanied by a decrease in DNA-binding level of the factor. Conclusions: Shikonin inhibits tumor invasion via downregulation of MMP-9 expression in ACC-M cells. Pharmacologic inhibition of the NF-kappa B-mediated MMP-9 expression by Shikonin might be a powerful treatment option for ACC patients in future.
资助项目	National Natural Science Fund of China[30772430] ; National Natural Science Fund of China[30801301]
WOS关键词	ADENOID CYSTIC CARCINOMA ; SALIVARY-GLAND CARCINOMAS ; TRANSCRIPTION FACTORS ; CANCER ; MATRIX-METALLOPROTEINASE-9 ; APOPTOSIS ; METASTASIS ; ACTIVATION ; PROMOTER ; ADHESION
WOS研究方向	Dentistry, Oral Surgery & Medicine
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000289253400003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278551]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Chenping, Z.
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Affiliated Peoples Hosp 9, Dept Oral & Maxillofacial Surg, Shanghai 200030, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Affiliated Peoples Hosp 1, Dept Stomatol, Shanghai 200030, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Med, Affiliated Peoples Hosp 9, Dept Oral Pathol, Shanghai 200030, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, SIBS, State Key Lab Drug Res, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Min, R.,Zun, Z.,Min, Y.,et al. Shikonin inhibits tumor invasion via down-regulation of NF-kappa B-mediated MMP-9 expression in human ACC-M cells[J]. ORAL DISEASES,2011,17(4):362-369.
APA	Min, R.,Zun, Z.,Min, Y.,Wenhu, D.,Wenjun, Y.,&Chenping, Z..(2011).Shikonin inhibits tumor invasion via down-regulation of NF-kappa B-mediated MMP-9 expression in human ACC-M cells.ORAL DISEASES,17(4),362-369.
MLA	Min, R.,et al."Shikonin inhibits tumor invasion via down-regulation of NF-kappa B-mediated MMP-9 expression in human ACC-M cells".ORAL DISEASES 17.4(2011):362-369.