Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR

doi:10.1073/pnas.1219446110

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	Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR
	Ji, Quanjiang 1; Zhang, Liang 1; Jones, Marcus B.4; Sun, Fei 1; Deng, Xin 1; Liang, Haihua 1; Cho, Hoonsik 2; Brugarolas, Pedro 1; Gao, Yihe N.1; Peterson, Scott N.4
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2013-03-26
卷号	110 期号:13 页码:5010-5015
关键词	thiol alkylation macrophage
ISSN号	0027-8424
DOI	10.1073/pnas.1219446110
文献子类	Article
英文摘要	Quinone molecules are intracellular electron-transport carriers, as well as critical intra-and extracellular signals. However, transcriptional regulation of quinone signaling and its molecular basis are poorly understood. Here, we identify a thiol-stress-sensing regulator YodB family transcriptional regulator as a central component of quinone stress response of Staphylococcus aureus, which we have termed the quinone-sensing and response repressor (QsrR). We also identify and confirm an unprecedented quinone-sensing mechanism based on the S-quinonization of the essential residue Cys-5. Structural characterizations of the QsrR-DNA and QsrR-menadione complexes further reveal that the covalent association of menadione directly leads to the release of QsrR from operator DNA following a 10 degrees rigid-body rotation as well as a 9-angstrom elongation between the dimeric subunits. The molecular level characterization of this quinone-sensing transcriptional regulator provides critical insights into quinone-mediated gene regulation in human pathogens.
资助项目	National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases[AI074658] ; NIH[P50GM081892]
WOS关键词	STAPHYLOCOCCUS-AUREUS ; BACILLUS-SUBTILIS ; REDOX SWITCH ; NAD(P)H-QUINONE REDUCTASE ; 2-ELECTRON REDUCTION ; ENTEROBACTER-CLOACAE ; 2-COMPONENT SYSTEM ; SENSING REGULATOR ; STRUCTURAL BASIS ; OXIDATION
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000318031900040
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277689]
专题	药理学第三研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	He, Chuan
作者单位	1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 2.Indiana Univ Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN 46408 USA; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Pathogen Funct Genom Resource Ctr, Infect Dis Grp, J Craig Venter Inst, Rockville, MD 20850 USA;
推荐引用方式 GB/T 7714	Ji, Quanjiang,Zhang, Liang,Jones, Marcus B.,et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(13):5010-5015.
APA	Ji, Quanjiang.,Zhang, Liang.,Jones, Marcus B..,Sun, Fei.,Deng, Xin.,...&He, Chuan.(2013).Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(13),5010-5015.
MLA	Ji, Quanjiang,et al."Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.13(2013):5010-5015.