A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling

doi:10.1074/mcp.M114.044255

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	A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling
	Wu, Zhixiang 1,2; Cheng, Zhongyi 3; Sun, Mingwei 1,2; Wan, Xuelian 1,2; Liu, Ping 1,2; He, Tieming 3; Tan, Minjia1,2 ; Zhao, Yingming1,2,4
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2015-02
卷号	14 期号:2 页码:329-339
ISSN号	1535-9476
DOI	10.1074/mcp.M114.044255
文献子类	Article
英文摘要	Methylation of lysine residues on histone proteins is known to play an important role in chromatin structure and function. However, non-histone protein substrates of this modification remain largely unknown. An effective approach for system-wide analysis of protein lysine methylation, particularly lysine monomethylation, is lacking. Here we describe a chemical proteomics approach for global screening for monomethyllysine substrates, involving chemical propionylation of monomethylated lysine, affinity enrichment of the modified monomethylated peptides, and HPLC/MS/MS analysis. Using this approach, we identified with high confidence 446 lysine monomethylation sites in 398 proteins, including three previously unknown histone monomethylation marks, representing the largest data set of protein lysine monomethylation described to date. Our data not only confirms previously discovered lysine methylation substrates in the nucleus and spliceosome, but also reveals new substrates associated with diverse biological processes. This method hence offers a powerful approach for dynamic study of protein lysine monomethylation under diverse cellular conditions and in human diseases.
资助项目	China Postdoctoral Science Foundation[2013M541567] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2012ZX09301001-007] ; National Basic Research Program of China (973 Program)[2014CBA02004] ; Natural Science Foundation of China[31370814] ; National Science AMP[00000000] ; Technology Major Project 'Key New Drug Creation and Manufacturing Program' of China[2014ZX09507-002] ; Shanghai Pujiang Program[13PJ1410300] ; NIH[GM105933] ; NIH[CA160036] ; NIH[RR020839] ; American Cancer Society[RSG-13-198-01-DDC]
WOS关键词	MASS-SPECTROMETRY ; POSTTRANSLATIONAL MODIFICATIONS ; PROTEIN METHYLATION ; INTERACTION NETWORKS ; HISTONE METHYLATION ; DRUG DISCOVERY ; IN-VIVO ; ACETYLATION ; METHYLTRANSFERASES ; ENRICHMENT
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000349144000006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276657]
专题	化学蛋白质组学研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zhao, Yingming
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China; 3.Jingjie PTM BioLab Hangzhou Co Ltd, Hangzhou, Zhejiang, Peoples R China; 4.Univ Chicago, Ben May Dept Canc Res, 924 E 57th St Knapp R120, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Wu, Zhixiang,Cheng, Zhongyi,Sun, Mingwei,et al. A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(2):329-339.
APA	Wu, Zhixiang.,Cheng, Zhongyi.,Sun, Mingwei.,Wan, Xuelian.,Liu, Ping.,...&Zhao, Yingming.(2015).A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling.MOLECULAR & CELLULAR PROTEOMICS,14(2),329-339.
MLA	Wu, Zhixiang,et al."A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling".MOLECULAR & CELLULAR PROTEOMICS 14.2(2015):329-339.