Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats

doi:10.1371/journal.pone.0135435

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	Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats
	Deng, Yanping 2; Yang, Min 2; Xu, Feng 3; Zhang, Qian 4; Zhao, Qun 2,3; Yu, Haitao 2; Li, Defang 1; Zhang, Ge 1; Lu, Aiping 1; Cho, Kenka 5
刊名	PLOS ONE
	2015-08-17
卷号	10 期号:8
ISSN号	1932-6203
DOI	10.1371/journal.pone.0135435
文献子类	Article
英文摘要	Lack of pharmacological strategies in clinics restricts the patient prognosis with myocardial ischemia/reperfusion (I/R) injury. The aim of this study was to evaluate the cardioprotection of combined salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1) against myocardial I/R injury and further investigate the underlying mechanism. I/R injury was induced by coronary artery ligation for Wistar male rats and hypoxia/reoxygenation injury was induced on H9c2 cells. Firstly, the best ratio between SalB and Rg1 was set as 2: 5 based on their effects on heart function detected by hemodynamic measurement. Then SalB-Rg1 (2: 5) was found to maintain mitochondrial membrane potential and resist apoptosis and necrosis in H9c2 cell with hypoxia/reoxygenation injury. Companying with same dose of SalB or Rg1 only, SalB-Rg1 showed more significant effects on down-regulation of myocardial infarct size, maintenance of myocardium structure, improvement on cardiac function, decrease of cytokine secretion including TNF-alpha, IL-1 beta, RANTES and sVCAM-1. Finally, the SalB-Rg1 improved the viability of cardiac myocytes other than cardiac fibroblasts in rats with I/R injury using flow cytometry. Our results revealed that SalB-Rg1 was a promising strategy to prevent myocardial I/R injury.
资助项目	National Science & Technology Major Project for "Key New Drug Creation and Manufacturing Program"[2013ZX09103002-024] ; National Natural Science Foundation of China[81173587] ; Shanghai Science and Technology Development Foundation[14401900900] ; Croucher Foundation[CAS14201] ; State Key Laboratory of Drug Research[SIMM1501KF-01]
WOS关键词	ISCHEMIC-REPERFUSION INJURY ; MYOCARDIAL-INFARCTION ; COMBINATION ; CELLS ; HEART ; ACTIVATION ; APOPTOSIS ; THERAPY ; MICE
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000359664300013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276436]
专题	上海中药现代化研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Jiang, Baohong
作者单位	1.Hong Kong Baptist Univ, Sch Chinese Med, Inst Adv Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China; 4.Fudan Univ, Sch Pharm, Dept Med Chem, Shanghai 200237, Peoples R China; 5.Takarazuka Univ Med & Hlth Care, Takarazuka, Hyogo 6660162, Japan
推荐引用方式 GB/T 7714	Deng, Yanping,Yang, Min,Xu, Feng,et al. Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats[J]. PLOS ONE,2015,10(8).
APA	Deng, Yanping.,Yang, Min.,Xu, Feng.,Zhang, Qian.,Zhao, Qun.,...&Jiang, Baohong.(2015).Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats.PLOS ONE,10(8).
MLA	Deng, Yanping,et al."Combined Salvianolic Acid B and Ginsenoside Rg1 Exerts Cardioprotection against Ischemia/Reperfusion Injury in Rats".PLOS ONE 10.8(2015).