A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1

doi:10.1016/j.bbagrm.2016.02.016

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	A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1
	Miao, Lingling 2; Yao, Hailan 1,4; Li, Chenggang 2; Pu, Mengfan 2; Yao, Xuan 1; Yang, Hui 1; Qi, Xinming2 ; Ren, Jin2 ; Wang, Yizheng 3
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
	2016-04
卷号	1859 期号:4 页码:650-662
关键词	MicroRNA Cytochrome P450 2E1 Gene regulation Transcription Translation
ISSN号	1874-9399
DOI	10.1016/j.bbagrm.2016.02.016
文献子类	Article
英文摘要	MicroRNAs (miRNAs) can direct post-transcriptional or transcriptional gene silencing. Here, we report that miR-552 is in the nucleus and cytosol and inhibits human cytochrome P450 (CYP) 2E1 expression at both transcriptional and post-transcriptional levels. MiR-552 via its non-seed sequence forms hybrids with a loop hairpin of the cruciform structure in CYP2E1 promoter region to inhibit SMARCE1 and RNA polymerase II binding to the promoter and CYP2E1 transcription. Expressing SMARCE1 reverses the inhibitory effects of miR-552 on CYP2E1 mRNA expression. MiR-552 with mutations in non-seed region losses its transcriptional, but retains its post transcriptional repression to CYP2E1. In contrast, mutation in miR-552 seed sequence suppresses its inhibitory effects on CYP2E1 expression at protein, but not at mRNA, levels. Our results suggest that miR-552 is a miRNA with a dual inhibitory ability at transcriptional and post-transcriptional levels leading to an effective inhibition. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81] ; National Natural Science Foundation of China[130] ; National Natural Science Foundation of China[081] ; National Science and Technology Major Project[2012ZX09302-003] ; National Science and Technology Major Project[2015ZX09102005]
WOS关键词	INDUCED LIVER-INJURY ; BREAST-CANCER CELLS ; POSTTRANSCRIPTIONAL REGULATION ; CYP2E1 EXPRESSION ; MAMMALIAN-CELLS ; SWI/SNF COMPLEX ; DNA METHYLATION ; GENE PROMOTERS ; TARGET ; PROTEIN
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000374370500011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276087]
专题	药物安全性评价中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ren, Jin; Wang, Yizheng
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neuroscience,State Key Lab Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Beijing Inst Basic Med Sci, Brain Sci Ctr, 27 Taiping Rd, Beijing 100850, Peoples R China; 4.Roskamp Inst, Ctr Adv Therapeut Strategies Brain Disorders, Sarasota, FL 34243 USA
推荐引用方式 GB/T 7714	Miao, Lingling,Yao, Hailan,Li, Chenggang,et al. A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,2016,1859(4):650-662.
APA	Miao, Lingling.,Yao, Hailan.,Li, Chenggang.,Pu, Mengfan.,Yao, Xuan.,...&Wang, Yizheng.(2016).A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1.BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS,1859(4),650-662.
MLA	Miao, Lingling,et al."A dual inhibition: microRNA-552 suppresses both transcription and translation of cytochrome P450 2E1".BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS 1859.4(2016):650-662.