Metabolic Regulation of Gene Expression by Histone Lysine beta-Hydroxybutyrylation

doi:10.1016/j.molcel.2016.03.036

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	Metabolic Regulation of Gene Expression by Histone Lysine beta-Hydroxybutyrylation
	Xie, Zhongyu 2; Zhang, Di 2; Chung, Dongjun 3,4; Tang, Zhanyun 5; Huang, He 2; Dai, Lunzhi 2; Qi, Shankang 2; Li, Jingya6 ; Colak, Gozde 2; Chen, Yue 2
刊名	MOLECULAR CELL
	2016-04-21
卷号	62 期号:2 页码:194-206
ISSN号	1097-2765
DOI	10.1016/j.molcel.2016.03.036
文献子类	Article
英文摘要	Here we report the identification and verification of a beta-hydroxybutyrate-derived protein modification, lysine beta-hydroxybutyrylation (Kbhb), as a new type of histone mark. Histone Kbhb marks are dramatically induced in response to elevated beta-hydroxybutyrate levels in cultured cells and in livers from mice subjected to prolonged fasting or streptozotocin-induced diabetic ketoacidosis. In total, we identified 44 histone Kbhb sites, a figure comparable to the known number of histone acetylation sites. By ChIP-seq and RNA-seq analysis, we demonstrate that histone Kbhb is a mark enriched in active gene promoters and that the increased H3K9bhb levels that occur during starvation are associated with genes upregulated in starvation-responsive metabolic pathways. Histone beta-hydroxybutyrylation thus represents a new epigenetic regulatory mark that couples metabolism to gene expression, offering a new avenue to study chromatin regulation and diverse functions of beta-hydroxybutyrate in the context of important human pathophysiological states, including diabetes, epilepsy, and neoplasia.
资助项目	NIH[DK089098] ; NIH[P01 DK057751] ; NIH[CT DPH 2014-0139] ; NIH[DK71900] ; NIH[R01GM101171] ; NIH[R21CA177925] ; NIH[GM59507] ; NIH[R01AG030593] ; NIH[R01AG023166] ; Ellison Medical Foundation[00000000] ; Starr Foundation Tri-Institutional Stem Cell Initiative[2014-021] ; National Natural Science Foundation of China[81125023] ; Shanghai Commission of Science and Technology[14431902800] ; National Basic Research Program of China (973 Program)[2014CBA02004] ; Shanghai Municipal Science and Technology Commission[15410723100] ; Nancy and Leonard Florsheim Family Fund[00000000]
WOS关键词	KETONE-BODIES ; HUMAN GENOME ; CHROMATIN ; EPIGENETICS ; IDENTIFICATION ; ACETYLATION ; PROMOTERS ; DISTINCT ; DISEASE ; STRESS
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000374643900006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276069]
专题	国家新药筛选中心中科院受体结构与功能重点实验室新药研究国家重点实验室化学蛋白质组学研究中心药物安全性评价中心
通讯作者	Yang, Xiaoyong; Zhao, Yingming
作者单位	1.Kyungpook Natl Univ, Plus KNU Multiom Based Creat Drug Res Team BK21, Coll Pharm, Daegu 41566, South Korea; 2.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA; 3.Yale Univ, Sch Publ Hlth, Dept Biostat, New Haven, CT 06520 USA; 4.Med Univ S Carolina, Dept Publ Hlth Sci, Charleston, SC 29425 USA; 5.Rockefeller Univ, Biochem & Mol Biol Lab, New York, NY 10065 USA; 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 7.Yale Univ, Sch Med, Comparat Med Sect, 333 Cedar St, New Haven, CT 06520 USA; 8.Yale Univ, Sch Med, Dept Cellular & Mol Physiol, 333 Cedar St, New Haven, CT 06520 USA; 9.Univ Chicago, Inst Genom & Syst Biol, Chicago, IL 60637 USA; 10.Univ Michigan, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA;
推荐引用方式 GB/T 7714	Xie, Zhongyu,Zhang, Di,Chung, Dongjun,et al. Metabolic Regulation of Gene Expression by Histone Lysine beta-Hydroxybutyrylation[J]. MOLECULAR CELL,2016,62(2):194-206.
APA	Xie, Zhongyu.,Zhang, Di.,Chung, Dongjun.,Tang, Zhanyun.,Huang, He.,...&Zhao, Yingming.(2016).Metabolic Regulation of Gene Expression by Histone Lysine beta-Hydroxybutyrylation.MOLECULAR CELL,62(2),194-206.
MLA	Xie, Zhongyu,et al."Metabolic Regulation of Gene Expression by Histone Lysine beta-Hydroxybutyrylation".MOLECULAR CELL 62.2(2016):194-206.