Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique

doi:10.1038/srep31795

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	Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique
	Chen, Lingsheng 2,3; Zhai, Linhui 3,4,5; Qu, Chunfeng 2,6; Zhang, Chengpu 3; Li, Sheng 2; Wu, Feilin 3; Qi, Yingzi 3; Lu, Fenghua 2; Xu, Ping 1,3,7; Li, Xiangping 2
刊名	SCIENTIFIC REPORTS
	2016-08-26
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep31795
文献子类	Article
英文摘要	To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome, and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation, and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during in vitro maturation.
资助项目	China High Technology Development program[2011AA100607] ; Guangxi Natural Science Funding[2012GXNSFCB053002] ; Guangxi Natural Science Funding[2014GXNSFAA118084]
WOS关键词	MOUSE OOCYTES ; MEIOTIC MATURATION ; EMBRYO DEVELOPMENT ; BOVINE OOCYTES ; TRANSCRIPTOME ANALYSIS ; EXPRESSION PROFILE ; PROTEIN EXPRESSION ; MAMMALIAN OOCYTES ; RNA LOCALIZATION ; RAT OOCYTES
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000381968100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275922]
专题	化学蛋白质组学研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Xu, Ping; Li, Xiangping; Shi, Deshun
作者单位	1.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China 2.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China; 3.Beijing Inst Radiat Med, Natl Ctr Prot Sci, Natl Engn Res Ctr Prot Drugs, State Key Lab Prote,Beijing Proteome Res Ctr, Beijing 102206, Peoples R China; 4.Chinese Acad Sci, Chem Prote Ctr, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 6.Hechi Peoples Hosp Guangxi, Dept Reprod Med, Hechi 547000, Peoples R China; 7.Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China;
推荐引用方式 GB/T 7714	Chen, Lingsheng,Zhai, Linhui,Qu, Chunfeng,et al. Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique[J]. SCIENTIFIC REPORTS,2016,6.
APA	Chen, Lingsheng.,Zhai, Linhui.,Qu, Chunfeng.,Zhang, Chengpu.,Li, Sheng.,...&Shi, Deshun.(2016).Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique.SCIENTIFIC REPORTS,6.
MLA	Chen, Lingsheng,et al."Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique".SCIENTIFIC REPORTS 6(2016).