Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo

doi:10.1124/jpet.105.093732

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第一研究室

	Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo
	Zhu, YN ; Zhao, WM; Yang, YF ; Liu, QF; Zhou, Y; Tian, J ; Ni, J ; Fu, YF ; Zhong, XG ; Tang, W
刊名	JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
	2006-02
卷号	316 期号:2 页码:662-669
ISSN号	0022-3565
DOI	10.1124/jpet.105.093732
文献子类	Article
英文摘要	Periploca sepium Bge, a traditional Chinese herb medicine, is used for treating rheumatoid arthritis in China. Followed the bioactivity-guided isolation, the most potent immunosuppressive compound, periplocoside E (PSE), a pregnane glycoside, had been identified from P. sepium Bge. We investigated the immunosuppressive effects of PSE in vitro and in vivo. The results showed that PSE in a dose-dependent manner significantly inhibited the proliferation of splenocytes induced by concanavalin A and mixed lymphocyte culture reaction at no cytotoxic concentrations (< 5 mu M). Administration of PSE suppressed a delayed-type hypersensitivity reaction, and ovalbumin ( OVA) induced antigen-specific immune responses in mice. In vivo treatment with PSE dose dependently suppressed OVA-induced proliferation and cytokine [interleukin (IL)-2 and interferon (IFN)-gamma] production from splenocytes in vitro. Purified T cells from OVA-immunized mice with PSE treatment showed its low ability for activation by OVA plus normal antigen presenting cell stimulation again in vitro. Further studies showed PSE dose dependently inhibited anti-CD3-induced primary T cell proliferation, activation for IL-2R alpha (CD25) expression, and cytokine (IFN-gamma and IL-2) production also at the transcriptional level. PSE was highly specific and significantly inhibited the activation of extracellular signal-regulated kinase and Jun N-terminal kinase, whereas activation of p38 was not affected in T cells stimulated with anti-CD3. These results demonstrated that PSE is an immunosuppressive compound in P. sepium Bge, which directly inhibits T cell activation in vitro and in vivo. This study provided evidence to understand the therapeutic effects of P. sepium Bge and indicated that this herb is appropriate for treatment of T cell-mediated disorders, such as autoimmune diseases.
WOS关键词	CHEMICAL-CONSTITUENTS ; ANTITUMOR FRACTION ; SIGNALING PATHWAYS ; PROTEIN-KINASES ; IL-2 PRODUCTION ; JNK ; ERK ; AUTOIMMUNE ; APOPTOSIS ; ARTHRITIS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
WOS记录号	WOS:000234759000021
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273678]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zuo, JP
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Grad Sch, Lab Nat Prod Chem, State Key Lab Drug Res,Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Zhu, YN,Zhao, WM,Yang, YF,et al. Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2006,316(2):662-669.
APA	Zhu, YN.,Zhao, WM.,Yang, YF.,Liu, QF.,Zhou, Y.,...&Zuo, JP.(2006).Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo.JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,316(2),662-669.
MLA	Zhu, YN,et al."Periplocoside E, an effective compound from Periploca sepium Bge, inhibited T cell activation in vitro and in vivo".JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 316.2(2006):662-669.