Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis

doi:10.1016/j.ejca.2006.07.008

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	Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis
	Li, Da-Qiang ; Hou, Yi-Feng ; Wu, Jiong ; Chen, Yi; Lu, Jin-Song ; Di, Gen-Hong ; Ou, Zhou-Luo ; Shen, Zhen-Zhou ; Ding, Jian; Shao, Zhi-Min
刊名	EUROPEAN JOURNAL OF CANCER
	2006-12
卷号	42 期号:18 页码:3274-3286
关键词	breast cancer metastasis gene expression profile ALL1-fused gene from chromosome 1q
ISSN号	0959-8049
DOI	10.1016/j.ejca.2006.07.008
文献子类	Article
英文摘要	To study the molecular mechanisms underlying breast cancer metastasis, gene expression profile analysis was performed on two well-established breast cancer cell lines with high and low metastatic potentials: MDA-MB-435HM and MDA-MB-435LM. The analysis was conducted using cDNA microarrays containing 8000 genes. Of 60 differentially expressed genes, ALL1-fused gene from chromosome 1q (AF1Q), a putative oncogene not described previously in breast cancer, was identified and found to be over-expressed in MDA-MB-435HM cells compared with MDA-MB-435LM cells. The results indicate that AF1Q may play an important role in breast cancer metastasis. To test this hypothesis, we generated an AF1Q high-expression cell line by stable transfection of AF1Q cDNA into MDA-MB-435LM cells. Results showed that over-expression of AF1Q led to a marked increase in the invasive and metastatic potential of MDA-MB-435LM cells in vitro and in vivo, accompanied by the up-regulation of matrix metalloproteinase-2 (MMP-2), MMP-9, transcription factor Ets-1, and RhoC expression in both mRNA and protein levels. Consistent with this observation, reduced AF1Q expression in MDA-MB-435HM cells by small interfering RNA (siRNA) resulted in a significant decrease in the invasive potential of MDA-MB-435HM cells in vitro and in the protein expression of MMP-2, MMP-9, Ets-1, and RhoC, compared with either parental or non-silencing control cells. These data provide functional evidence that oncogene AF1Q may be a novel mediator of metastasis promotion in human breast cancer through regulation of the MMP pathway and RhoC expression. (c) 2006 Elsevier Ltd. All rights reserved.
WOS关键词	MAMMARY EPITHELIAL-CELLS ; IN-VIVO ; TRANSCRIPTION FACTOR ; CARCINOMA-CELLS ; ACUTE-LEUKEMIA ; PROGRESSION ; RHOC ; INVASION ; METALLOPROTEINASES ; CLASSIFICATION
WOS研究方向	Oncology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000243304900035
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273433]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Shao, Zhi-Min
作者单位	1.Fudan Univ, Canc Hosp, Inst Canc, Dept Breast Surg,Breast Canc Inst, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Anti Tumor Pharmacol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Li, Da-Qiang,Hou, Yi-Feng,Wu, Jiong,et al. Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis[J]. EUROPEAN JOURNAL OF CANCER,2006,42(18):3274-3286.
APA	Li, Da-Qiang.,Hou, Yi-Feng.,Wu, Jiong.,Chen, Yi.,Lu, Jin-Song.,...&Shao, Zhi-Min.(2006).Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis.EUROPEAN JOURNAL OF CANCER,42(18),3274-3286.
MLA	Li, Da-Qiang,et al."Gene expression profile analysis of an isogenic tumour metastasis model reveals a functional role for oncogene AF1Q in breast cancer metastasis".EUROPEAN JOURNAL OF CANCER 42.18(2006):3274-3286.