7 '-(3 ',4 '-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo

doi:10.1016/j.lfs.2007.10.004

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	7 '-(3 ',4 '-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo
	Hu, Xu-Dong 2; Zhong, Xiang-Gen 3; Zhang, Xiang-Hua 1; Zhang, Yi-Nan 1; Zheng, Zong-Ping 1; Zhou, Yu3 ; Tang, Wei3 ; Yang, Yang 2; Yang, Yi-Fu 3; Hu, Li-Hong 1
刊名	LIFE SCIENCES
	2007-12-14
卷号	81 期号:25-26 页码:1677-1684
关键词	Z23 splenocyte proliferation mixed lymphocyte culture reaction delayed-type hypersensitivity collagen-induced arthritis
ISSN号	0024-3205
DOI	10.1016/j.lfs.2007.10.004
文献子类	Article
英文摘要	Fissistigma oldhamii (Hemsl.) Merr [F oldhamii], a traditional Chinese herb medicine, is widely used for treating rheumatoid arthritis (RA) in China. Following bioactivity-guided isolation, a representative immunosuppressive compound with low cytotoxicity, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), was been identified in this herb medicine. We investigated the immunosuppressive effects of Z23 on T cells in vitro and in vivo. The results showed that Z23 in a dose-dependent manner significantly inhibited the proliferation of splenocytes induced by concanavalin A (ConA) and by the mixed lymphocyte culture reaction (MLR), with half inhibitive concentration (IC50) values of 6.22 mu M and 0.78 mu M, respectively. Z23 also dose-dependently inhibited the proliferation and type 1 cytokine (IFN-gamma and IL-2) production of primary T cells stimulated by anti-CD3/CD28 mAbs, but did not affect IL-12 production by mouse peritoneal macrophages (pM phi) stimulated with LPS plus IFN-gamma in vitro. Administration of Z23 (6.25 mg/kg, 12.5 mg/kg, 25 mg/kg, i.p.) dose-dependently suppressed 2,4-dinitrofluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) reactions. Furthermore, administration of Z23 (25 mg/kg, i.p.) significantly reduced the incidence and severity of type II bovine collagen (CII)-induced arthritis (CIA), which was associated with the inhibition of CII-specific T cell proliferation and type 1 cytokine (IFN-gamma and IL-2) production. In this study, we report that a representative immunosuppressive compound from F oldhamii, Z23, effectively inhibits murine immune responses in vitro and in vivo, and that the immunosuppressive effects of Z23 might be attributed to suppression of T cell activation and function and Th1 type cytokine production. (C) 2007 Elsevier Inc. All rights reserved.
WOS关键词	DELAYED-TYPE HYPERSENSITIVITY ; COLLAGEN-INDUCED ARTHRITIS ; RHEUMATOID-ARTHRITIS ; ANTIGEN ; PROLIFERATION ; AUTOIMMUNITY ; RESVERATROL ; MECHANISMS ; EXPRESSION ; INDUCTION
WOS研究方向	Research & Experimental Medicine ; Pharmacology & Pharmacy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000251990200007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273093]
专题	上海中药现代化研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Hu, Li-Hong
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; 2.Shanghai Univ Tradit Chinese Med, Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Hu, Xu-Dong,Zhong, Xiang-Gen,Zhang, Xiang-Hua,et al. 7 '-(3 ',4 '-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo[J]. LIFE SCIENCES,2007,81(25-26):1677-1684.
APA	Hu, Xu-Dong.,Zhong, Xiang-Gen.,Zhang, Xiang-Hua.,Zhang, Yi-Nan.,Zheng, Zong-Ping.,...&Zuo, Jian-Ping.(2007).7 '-(3 ',4 '-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo.LIFE SCIENCES,81(25-26),1677-1684.
MLA	Hu, Xu-Dong,et al."7 '-(3 ',4 '-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo".LIFE SCIENCES 81.25-26(2007):1677-1684.