Crystal structure of HAb18g/CD147 - Implications for immunoglobulin superfamily homophilic adhesion

doi:10.1074/jbc.M802694200

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	Crystal structure of HAb18g/CD147 - Implications for immunoglobulin superfamily homophilic adhesion
	Yu, Xiao-Ling 2,3; Hu, Tiancen 4; Du, Jia-Mu 5; Ding, Jian-Ping 5; Yang, Xiang-Min 2,3; Zhang, Jian 4; Yang, Bin 2,3; Shen, Xu4 ; Zhang, Zheng 2,3; Zhong, Wei-De 6
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2008-06-27
卷号	283 期号:26 页码:18056-18065
ISSN号	0021-9258
DOI	10.1074/jbc.M802694200
文献子类	Article
英文摘要	CD147, a member of the immunoglobulin superfamily (IgSF), plays fundamental roles in intercellular interactions in numerous pathological and physiological processes. Importantly, our previous studies have demonstrated that HAb18G/CD147 is a novel hepatocellular carcinoma (HCC)-associated antigen, and HAb18G/CD147 stimulates adjacent fibroblasts and HCC cells to produce elevated levels of several matrix metalloproteinases, facilitating invasion and metastasis of HCC cells. In addition, HAb18G/CD147 has also been shown to be a novel universal cancer biomarker for diagnosis and prognostic assessment of a wide range of cancers. However, the structural basis underlying the multifunctional character of CD147 remains unresolved. We report here the crystal structure of the extracellular portion of HAb18G/CD147 at 2.8 angstrom resolution. The structure comprises an N-terminal IgC2 domain and a C-terminal IgI domain, which are connected by a 5-residue flexible linker. This unique C2-I domain organization is distinct from those of other IgSF members. Four homophilic dimers exist in the crystal and adopt C2-C2 and C2-I dimerization rather than V-V dimerization commonly found in other IgSF members. This type of homophilic association thus presents a novel model for homophilic interaction between C2 domains of IgSF members. Moreover, the crystal structure of HAb18G/CD147 provides a good structural explanation for the established multifunction of CD147 mediated by homo/heterooligomerizations and should represent a general architecture of other CD147 family members.
WOS关键词	MATRIX-METALLOPROTEINASE INDUCER ; CELL-SURFACE EXPRESSION ; MOLECULAR-DYNAMICS ; BINDING FRAGMENT ; SOLUBLE FORM ; RESOLUTION ; BASIGIN ; ANTIGEN ; CD147 ; RECOGNITION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000256949200035
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272887]
专题	药物发现与设计中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zhu, Ping
作者单位	1.Fourth Mil Med Univ, Xijing Hosp, Dept Clin Immunol, Xian 710032, Peoples R China; 2.Fourth Mil Med Univ, State Key Lab Canc Biol, Dept Cell Biol, Xian 710032, Peoples R China; 3.Fourth Mil Med Univ, Cell Engn Res Ctr, Xian 710032, Peoples R China; 4.Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Drug Discovery & Design Ctr, Shanghai 201203, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China; 6.Guangzhou First Municipal Peoples Hosp, Guangzhou Med Coll, Guangzhou 510182, Peoples R China; 7.Chinese Peoples Liberat Army Gen Hosp, Beijing 100853, Peoples R China
推荐引用方式 GB/T 7714	Yu, Xiao-Ling,Hu, Tiancen,Du, Jia-Mu,et al. Crystal structure of HAb18g/CD147 - Implications for immunoglobulin superfamily homophilic adhesion[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2008,283(26):18056-18065.
APA	Yu, Xiao-Ling.,Hu, Tiancen.,Du, Jia-Mu.,Ding, Jian-Ping.,Yang, Xiang-Min.,...&Chen, Zhi-Nan.(2008).Crystal structure of HAb18g/CD147 - Implications for immunoglobulin superfamily homophilic adhesion.JOURNAL OF BIOLOGICAL CHEMISTRY,283(26),18056-18065.
MLA	Yu, Xiao-Ling,et al."Crystal structure of HAb18g/CD147 - Implications for immunoglobulin superfamily homophilic adhesion".JOURNAL OF BIOLOGICAL CHEMISTRY 283.26(2008):18056-18065.