Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.

doi:10.3389/fphar.2018.00629

	Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.
	Li, Xun 2,3; Qiu, Zidong 2,3; Jin, Qinghao 1,2; Chen, Guilin 2,4; Guo, Mingquan 2,4
刊名	FRONTIERS IN PHARMACOLOGY
	2018-06-12
卷号	9
关键词	colon cancer Toddalia asiatica Lam. G2/M arrest apoptosis reactive oxygen species
ISSN号	1663-9812
DOI	10.3389/fphar.2018.00629
文献子类	Article
英文摘要	The roots of Toddalia asiatica (L.) Lam. (TA) has been often used in Chinese folk medicine to treat different diseases, including but not limited to arthritis, injuries, stomachache, and even tumors. However, the anti-cancer effects and the action mechanisms of TA remain elusive. Therefore, we firstly evaluated the effects of different extracts of TA on the growth of human colon cancer cells, and then tried to further elucidate their underlying molecular mechanisms. As a result, the dichloromethane fraction (DF) was found to possess the highest anti-proliferative activity with IC50 value at 18 mu g/mL among all of the four extracts from TA, and strongly inhibited HT-29 cell growth and halted cell cycle progression in G2/M phase. DF also induced phosphatidylserine externalization and activated caspases -8, -9, and -3, suggesting DF induced apoptosis through intrinsic and extrinsic pathways. Furthermore, we found that HT-29 cell cycle arrest induced by DF could be the result of reactive oxygen species (ROS), as the ROS scavenger N-acetyl cysteine (NAC) attenuating it. Taken together, these results indicated that DF induced cell cycle arrest at G2/M phase and apoptosis in HT-29 cells, and could be a promising source for developing natural therapeutics for colon cancer.
资助项目	Natural Science Foundation of China[81673580] ; "One Hundred Talents Project" from the Chinese Academy of Sciences[29Y429291a0129] ; Major Project for Special Technology Innovation of Hubei Province - the Sinoforeign Cooperation Project[2017AHB054]
WOS关键词	CANCER-THERAPY ; ROOT EXTRACT ; CYTOTOXICITY ; MECHANISMS ; DEATH ; DIFFERENTIATION ; CONSTITUENTS ; INDUCTION ; COUMARIN ; PATHWAYS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000435097200001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279710]
专题	中国科学院上海药物研究所
通讯作者	Guo, Mingquan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China; 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Xun,Qiu, Zidong,Jin, Qinghao,et al. Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.[J]. FRONTIERS IN PHARMACOLOGY,2018,9.
APA	Li, Xun,Qiu, Zidong,Jin, Qinghao,Chen, Guilin,&Guo, Mingquan.(2018).Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam..FRONTIERS IN PHARMACOLOGY,9.
MLA	Li, Xun,et al."Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.".FRONTIERS IN PHARMACOLOGY 9(2018).