Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1

doi:10.1016/j.bbrc.2011.04.088

	Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1
	Lei, Jian 1; Mesters, Jeroen R.1; von Brunn, Albrecht 2; Hilgenfeld, Rolf 1,3,4
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2011-05-20
卷号	408 期号:4 页码:680-685
关键词	PABP Paip1 HEAT domain Eukaryotic translation initiation factors X-ray structure
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2011.04.088
文献子类	Article
英文摘要	In eukaryotes, the poly(A)-binding protein (PABP) is one of the important factors for initiation of messenger RNA translation. PABP activity is regulated by the PABP-interacting proteins (Paips), which include Paip1, Paip2A, and Paip2B. Human Paip1 has three different isoforms. Here, we report the crystal structure of the middle domain of Paip1 isoform 2 (Paipl M) as determined by single-wavelength anomalous dispersion phasing. The structure reveals a crescent-shaped domain consisting of 10 alpha-helices and two antiparallel beta-strands forming a beta-hairpin. The 10 alpha-helices are arranged as five HEAT repeats which form a double layer of alpha helices with a convex and a concave surface. Despite low sequence identity, the overall fold of Paipl M is similar to the middle domain of human eIF4GII and yeast eIF4GI. Moreover, the amino-acid sequence motif and the local structure of eIF4G involved in binding of eIF4A, are conserved in Paipl. The structure reported here is the first of a member of the Paip family, thereby filling a gap in our understanding of initiation of eukaryotic mRNA translation in three dimensions. (C) 2011 Elsevier Inc. All rights reserved.
资助项目	Helmholtz Zentrum Berlin fur Materialien und Energie[00000000] ; Freie Universitat Berlin[00000000] ; Humboldt-Universitat zu Berlin[00000000] ; Max-Delbruck Centrum[00000000] ; Leibniz-lnstitut fur Molekulare Pharmakologie[00000000] ; DFG[00000000] ; Fonds der Chemischen Industrie[00000000] ; Chinese Academy of Sciences (CAS)[2010T1S6] ; BMBF[01KIO701]
WOS关键词	TRANSLATION INITIATION-FACTORS ; POLY(A) BINDING-PROTEIN ; MESSENGER-RNA ; EUKARYOTIC TRANSLATION ; SEQUENCE ; MODEL ; PABP
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000291293200031
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278522]
专题	中国科学院上海药物研究所
通讯作者	Hilgenfeld, Rolf
作者单位	1.Univ Lubeck, Inst Biochem, Ctr Struct & Cell Biol Med, D-23538 Lubeck, Germany; 2.Univ Munich, Max Von Pettenkofer Inst, Lehrstuhl Virol, D-80336 Munich, Germany; 3.DESY, Lab Struct Biol Infect & Inflammat, D-22603 Hamburg, Germany; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Lei, Jian,Mesters, Jeroen R.,von Brunn, Albrecht,et al. Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,408(4):680-685.
APA	Lei, Jian,Mesters, Jeroen R.,von Brunn, Albrecht,&Hilgenfeld, Rolf.(2011).Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,408(4),680-685.
MLA	Lei, Jian,et al."Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 408.4(2011):680-685.