SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells

doi:10.1159/000354458

	SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells
	Tan, Ming-Yue 1; Mu, Xing-Yu 1; Liu, Bin 2; Wang, Yong 1; Bao, Er-Dun 1; Qiu, Jian-Xin 1; Fan, Yu 1
刊名	CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
	2013
卷号	32 期号:3 页码:542-548
关键词	SENP2 Metastasis MMP13 Bladder cancer cells
ISSN号	1015-8987
DOI	10.1159/000354458
文献子类	Article
英文摘要	Background: SUMO-specific protease 2 (SENP2) is a de-SUMOylation protease family member which has an indispensable role in the regulation of NF-B-K transcriptional activation and Wnt signaling. However, whether SENP2 plays a role in tumor metastasis is completely unknown. Methods: Real-time PCR and Western blot was used to detect the expression of SENP2 in human bladder cancer samples and cell lines. Small interfering RNA (siRNA) was used to silencing the expression of SENP2. Matrigel-coated invasion chambers were used to detect the invasion ability of SENP2 in bladder cancer cells. Results: SENP2 was downregulated in bladder cancer samples. SENP2 inhibited bladder cancer cells migration and invasion in vitro. Transcriptional analysis of several genes associated with tumor metastasis and invasion demonstrated that SENP2 selectively down-regulated MMP13 in bladder cancer cells. Further analysis indicated that silencing of MMP13 rescued the invasive phenotype in SENP2 expressing T24 cells. Conclusion: SENP2 functions as a tumor metastasis suppressor in bladder cancer. The effects of SENP2 on bladder cancer invasion are partially mediated by inhibiting the expression of MMP13. Copyright (C) 2013 S. Karger AG, Basel
WOS关键词	EMERGING MECHANISMS ; METASTASIS ; SUMOYLATION ; PROGRESSION ; ROLES
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	KARGER
WOS记录号	WOS:000325415900005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277786]
专题	中国科学院上海药物研究所
通讯作者	Fan, Yu
作者单位	1.Shanghai Jiao Tong Univ, Dept Urol, Affiliated Peoples Hosp 1, Shanghai 200080, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Tan, Ming-Yue,Mu, Xing-Yu,Liu, Bin,et al. SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2013,32(3):542-548.
APA	Tan, Ming-Yue.,Mu, Xing-Yu.,Liu, Bin.,Wang, Yong.,Bao, Er-Dun.,...&Fan, Yu.(2013).SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,32(3),542-548.
MLA	Tan, Ming-Yue,et al."SUMO-Specific Protease 2 Suppresses Cell Migration and Invasion through Inhibiting the Expression of MMP13 in Bladder Cancer Cells".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 32.3(2013):542-548.