CHARACTERIZATION OF A FLUOROURACIL-RESISTANT HUMAN GASTRIC-CARCINOMA CELL-LINE AND ITS MORPHOLOGICAL BEHAVIOR

	CHARACTERIZATION OF A FLUOROURACIL-RESISTANT HUMAN GASTRIC-CARCINOMA CELL-LINE AND ITS MORPHOLOGICAL BEHAVIOR
	YUE, XF; HAN, JX; DAI, ZQ; LIU, MZ; YANG, WY; XU, JY; ZHOU, JJ; CHEN, LJ; ZHANG, SY
刊名	ACTA PHARMACOLOGICA SINICA
	1993-11
卷号	14 页码:S1-S4
关键词	FLUOROURACIL STOMACH NEOPLASMS CULTURED TUMOR CELLS DRUG RESISTANCE NUDE MICE
ISSN号	0253-9756
文献子类	Article
英文摘要	A fluorouracil (FU)-resistant human carcinoma cell line (SGC-7901/R) was established in culture by progressively increasing the concentration of FU from 50 ng . ml-1 to 2. 5 mg . ml-1. The cell line has been successfully subcultured for more than 150 passages during more than 2 years. Its degree of FU resistance was 139-fold vs that of FU sensitive cell line (SGC-7901/S), and the resistant phenotype was stable when cells were cultured for 23 passages in FU-free medium. The doubling time was 17.3 and 25.6 h for resistant cells and parental cells , respectively. Swiss (nu/nu) nude mice were used for the in vivo experiment, the FU-resistant cell line also exhibited resistance to FU and cross-resistance to mitomycin C. FU inhibited markedly the incorporation of [H-3]UR into sensitive cells and only showed a 31.6% inhibition with FU 100 mug . ml-1 in resistant cells. For the incorporation of [H-3]TdR into DNA, inhibitory rates were seen with different concentrations of FU in resistant cells. By morphologic observation, SGC-7901/R cells showed little secretion but without any tendency to glandular pattern. Their nuclei were allotype with enlarged perinuclear space and a few intranuclear pseudoinclusions. The mitotic phase of cells was found frequently. The phenotype of resistant cell line can be deduced more malignant than that of parental cell line.
WOS关键词	COLORIMETRIC ASSAY ; 5-FLUOROURACIL
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	日语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:A1993MH44400001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275121]
专题	中国科学院上海药物研究所
通讯作者	YUE, XF
作者单位	CHINESE ACAD SCI,SHANGHAI INST MAT MED,SHANGHAI 200031,PEOPLES R CHINA.
推荐引用方式 GB/T 7714	YUE, XF,HAN, JX,DAI, ZQ,et al. CHARACTERIZATION OF A FLUOROURACIL-RESISTANT HUMAN GASTRIC-CARCINOMA CELL-LINE AND ITS MORPHOLOGICAL BEHAVIOR[J]. ACTA PHARMACOLOGICA SINICA,1993,14:S1-S4.
APA	YUE, XF.,HAN, JX.,DAI, ZQ.,LIU, MZ.,YANG, WY.,...&ZHANG, SY.(1993).CHARACTERIZATION OF A FLUOROURACIL-RESISTANT HUMAN GASTRIC-CARCINOMA CELL-LINE AND ITS MORPHOLOGICAL BEHAVIOR.ACTA PHARMACOLOGICA SINICA,14,S1-S4.
MLA	YUE, XF,et al."CHARACTERIZATION OF A FLUOROURACIL-RESISTANT HUMAN GASTRIC-CARCINOMA CELL-LINE AND ITS MORPHOLOGICAL BEHAVIOR".ACTA PHARMACOLOGICA SINICA 14(1993):S1-S4.