Dopamine-glutamate interaction in rat striatal slices: changes of CCDPK II, PKA, and LDH activity by receptor-mediated mechanisms

	Dopamine-glutamate interaction in rat striatal slices: changes of CCDPK II, PKA, and LDH activity by receptor-mediated mechanisms
	Tang, FM ; Sun, YF ; Wang, R ; Ding, YM ; Zhang, GY ; Jin, GZ
刊名	ACTA PHARMACOLOGICA SINICA
	2000-02
卷号	21 期号:2 页码:145-150
关键词	dopamine glutamates dopamine receptors N-methyl-D-aspartate receptors corpus striatum Ca2+-calmodulin dependent protein kinase cyclic AMP-dependent protein kinases lactate dehydrogenase
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To Study the effects of dopamine (DA) and glutamate (Glu) and their receptor agonists/antagonists on Ca2+/calmodulin-dependent protein kinase IT (CCDPK II), cyclic AMP-dependent protein kinase A (PKA) activities and the:LDH release in rat striatal slices, and to examine the interaction between DA and Glu transmitter systems in: striatum. METHODS: The activities of CCDPK II, PKA, and the release of LDH were determined with the (32) P-incorporation and colorimetry respectively in rat striatal slices. RESULTS: (1) Exogenous DA, D-1 receptor agonist SKF 38393 and D-2 receptor agonist LY 171555 reduced CCDPK II activity in striatal slices;:Glu also inhibited CCPPK II activity in a concentration-dependent manner. NMDA receptor antagonist MK-801 could-antagonize the inhibitory effect of SKF 38393 and:LY171555 on the CCDPK II activity. DI and D-2 receptor antagonists SCH 23390 and spiperone could also antagonize the decrease of CCDPK II activity induced by Glu; (2) DA and SKF 38393 markedly increased PKA activity in striatal slices, which was reduced by MK-801; (3) DA and Glu increased the release of LDH from the striatal neurons in a concentration-dependent manner. MK-801 antagonized the increase of LDH induced by DA. Spiperone, rather than SCH 23390, could reduce the release of LDH from striatal neuron in the presence of Glu. CONCLUSION: The interaction between DA and Glu transmitter systems is found in the regulation of the CCDPK Il and PKA activities and cell function in the striatum.
WOS关键词	PROTEIN KINASE-II ; CEREBRAL-ISCHEMIA ; DAMAGE ; HYPOXIA ; NEURONS ; CULTURE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000085208200007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274645]
专题	中国科学院上海药物研究所
通讯作者	Tang, FM
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Xuzhou Med Coll, Res Ctr Biochem & Mol Biol, Xuzhou 221002, Peoples R China
推荐引用方式 GB/T 7714	Tang, FM,Sun, YF,Wang, R,et al. Dopamine-glutamate interaction in rat striatal slices: changes of CCDPK II, PKA, and LDH activity by receptor-mediated mechanisms[J]. ACTA PHARMACOLOGICA SINICA,2000,21(2):145-150.
APA	Tang, FM,Sun, YF,Wang, R,Ding, YM,Zhang, GY,&Jin, GZ.(2000).Dopamine-glutamate interaction in rat striatal slices: changes of CCDPK II, PKA, and LDH activity by receptor-mediated mechanisms.ACTA PHARMACOLOGICA SINICA,21(2),145-150.
MLA	Tang, FM,et al."Dopamine-glutamate interaction in rat striatal slices: changes of CCDPK II, PKA, and LDH activity by receptor-mediated mechanisms".ACTA PHARMACOLOGICA SINICA 21.2(2000):145-150.