Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation

doi:10.1111/j.1745-7254.2006.00254.x

	Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation
	He, Z ; Li, JJ ; Zhen, CH ; Feng, LY; Ding, XY
刊名	ACTA PHARMACOLOGICA SINICA
	2006-01
卷号	27 期号:1 页码:80-90
关键词	leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies
ISSN号	1671-4083
DOI	10.1111/j.1745-7254.2006.00254.x
文献子类	Article
英文摘要	Aim: Leukemia inhibitory factor (LIF), a pleiotropic cytokine, has been used extensively in the maintenance of mouse embryonic stem cell pluripotency. In this current work, we examined the effect of the LIF signaling pathway in embryonic stem (ES) cell differentiation to a neural fate. Methods: In the presence of LIF (1000 U/mL), the production of neuronal cells derived from embryoid bodies (EB) was tested under various culture conditions. Inhibition of the LIF pathway was examined with specific inhibitors. The effects of cell apoptosis and proliferation on neural differentiation were examined. ES cell differentiation into three-germ layers was compared. Results: Under various culture conditions, neuronal differentiation was increased in the presence of LIF. Blocking the LIF-activated STAT3 signaling pathway with specific inhibitors abolished the neuronal differentiation of ES cells, whereas inhibition of the LIF-activated MEK signaling pathway impaired the differentiation of ES cells toward a glial fate. LIF suppressed cell apoptosis and promoted cell proliferation during ES cell differentiation. LIF inhibited the differentiation of ES cells to both mesoderm and extraembryonic endoderm fates, but enhanced the determination of neural progenitors. Conclusion: These results suggest that LIF plays a positive role during the differentiation of ES cells into neuronal cells.
WOS关键词	IN-VITRO DIFFERENTIATION ; SELF-RENEWAL ; NEURAL INDUCTION ; ES CELLS ; SIGNALING PATHWAY ; RETINOIC ACID ; MUSCLE CELLS ; STAT3 ; ACTIVATION ; SPECIFICATION
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:2659589
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000234427200008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273693]
专题	中国科学院上海药物研究所
通讯作者	Ding, XY
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cell Biol, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Stem Cell Biol, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	He, Z,Li, JJ,Zhen, CH,et al. Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation[J]. ACTA PHARMACOLOGICA SINICA,2006,27(1):80-90.
APA	He, Z,Li, JJ,Zhen, CH,Feng, LY,&Ding, XY.(2006).Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation.ACTA PHARMACOLOGICA SINICA,27(1),80-90.
MLA	He, Z,et al."Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation".ACTA PHARMACOLOGICA SINICA 27.1(2006):80-90.