Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine

doi:10.1111/j.1745-7254.2007.00569.x

	Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine
	Jin, Wei ; Qu, Le-feng ; Chen, Qin ; Chang, Xin-zhong ; Wu, Jiong ; Shao, Zhi-min
刊名	ACTA PHARMACOLOGICA SINICA
	2007-06
卷号	28 期号:6 页码:859-868
关键词	QGY-7703 cells homoharringtonine apoptosis gene expression
ISSN号	1671-4083
DOI	10.1111/j.1745-7254.2007.00569.x
文献子类	Article
英文摘要	Aim: To classify the genes responsible for apoptosis in QGY-7703 cells induced by homoharringtonine (HHT). Methods: Apoptosis in QGY-7703 cells induced by HHT was demonstrated by DNA fragmentation and morphological observation. cDNA microarray technology was used to detect gene transcription, and the result of microarrays for genes was confirmed by RT-PCR. Results: Seventy-eight individual mRNA were identified and their transcription levels changed significantly. Those genes, of which 68% were upregulated and 32% were downregulated, were partially related to apoptosis. They were mostly oncogenes, tumor suppressors, enzymes, and kinases. Conclusion: HHT is a potential drug in the treatment of liver cancer. TGF-beta, TNF, FAS, p38 MAP K, and p53 apoptosis signaling pathways were activated during apoptosis in QGY-7703 cells. Such inducible genes may play important roles in apoptosis and deserve to be further studied.
WOS关键词	TRANSCRIPTION FACTOR ; NEURONAL APOPTOSIS ; OXIDATIVE STRESS ; KAPPA-B ; PROTEIN ; OVEREXPRESSION ; IDENTIFICATION ; INHIBITOR ; DEATH ; LOCALIZATION
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:2845678
出版者	BLACKWELL PUBLISHING
WOS记录号	WOS:000246756300014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273235]
专题	中国科学院上海药物研究所
通讯作者	Shao, Zhi-min
作者单位	1.Fudan Univ, Inst Biomed Sci, Shanghai Med Coll, Dept Oncol,Breast Canc Inst,Canc Hosp, Shanghai 200032, Peoples R China 2.Changhai Hosp, Dept Vasc Surg, PLA, Shanghai 200433, Peoples R China 3.Changhai Hosp, Inst Vasc Surg, PLA, Shanghai 200433, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Jin, Wei,Qu, Le-feng,Chen, Qin,et al. Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine[J]. ACTA PHARMACOLOGICA SINICA,2007,28(6):859-868.
APA	Jin, Wei,Qu, Le-feng,Chen, Qin,Chang, Xin-zhong,Wu, Jiong,&Shao, Zhi-min.(2007).Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine.ACTA PHARMACOLOGICA SINICA,28(6),859-868.
MLA	Jin, Wei,et al."Gene expression pattern in apoptotic QGY-7703 cells induced by homoharringtonine".ACTA PHARMACOLOGICA SINICA 28.6(2007):859-868.