Experimental study of differentiation and growth suppression of Wilms' tumor in response to All-trans-retinoic acid

	Experimental study of differentiation and growth suppression of Wilms' tumor in response to All-trans-retinoic acid
	He Xiaowei 1; Gao Jiechun 1; Yuan Shengtoa 2
刊名	Chinese Journal of Pediatric Surgery
	2003
卷号	24 期号:5 页码:455-458
关键词	Nephroblastoma Disease models, animal Tretinoin
ISSN号	0253-3006
其他题名	维甲酸对肾母细胞瘤分化诱导和生长抑制的实验研究
文献子类	Article
英文摘要	Objective To study All-trans-retinoic acid induced differentiation and growth suppression of Wilms tumor in vivo. Methods An orthotopic xenografts model of human Wilms' tumor was developed in nude mice. Randomly, a total of 30 nude mice bearing orthotopic xenografts of human Wilms' tumor were equally divided into experimental and control groups. The experimental group was administered with 30 mg/ kg·d retinoic acid dissolved in 0.1 ml .sesame oil starting 3 weeks after tumor transplantation, and the control group received equivalent volume of sesame oil . The weight of transplanted tumors and their host nude mice were directly measured. Proliferation index (PI) was evaluated in transplanted tumor by FCM. Also, transplanted tumor was subjected to light microscopic observation on morphological differentiation and RT-PCR amplification for insulin- like growth factor 2 (IGF2) and HI9 niRNA. Results No significant difference was detected in body weight of mice between the experimental and control ( P＞ 0.05). The transplanted tumors derived from nude mice of experimental group increased in weight less than those from control (P<0.05). PI of the experimental group (17.82.8) was also lower than that from control (20.82.1, P<0.05). Moreover, better morphological differentiation appearing could be noted in experimental group under electronic microscope. IGF2 mRNA was amplified in transplanted tumor but no significant difference was found in semiquantitative value of RT-PCR products between the two groups. H19 transcripts were negative amplification in all tumor samples. Conclusion Retinoic acid plays a role in inhibition Wilms' tumors from growth as xenografts in nude mice by inducing differentiation, which has no direct linkage with regulation of IGF2 and HI9 transcription.
WOS研究方向	Pediatrics (provided by Clarivate Analytics)
语种	中文
CSCD记录号	CSCD:1326078
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/268426]
专题	中国科学院上海药物研究所
作者单位	1.Department of Pediatric Surgery, Shanghai 200032, China.; 2.Children Hospital of Fudan University, Shanghai 200032, China.
推荐引用方式 GB/T 7714	He Xiaowei,Gao Jiechun,Yuan Shengtoa. Experimental study of differentiation and growth suppression of Wilms' tumor in response to All-trans-retinoic acid[J]. Chinese Journal of Pediatric Surgery,2003,24(5):455-458.
APA	He Xiaowei,Gao Jiechun,&Yuan Shengtoa.(2003).Experimental study of differentiation and growth suppression of Wilms' tumor in response to All-trans-retinoic acid.Chinese Journal of Pediatric Surgery,24(5),455-458.
MLA	He Xiaowei,et al."Experimental study of differentiation and growth suppression of Wilms' tumor in response to All-trans-retinoic acid".Chinese Journal of Pediatric Surgery 24.5(2003):455-458.